Fluorescent probes with high pKa values based on traditional, near-infrared rhodamine, and hemicyanine fluorophores for sensitive detection of lysosomal pH variations.
Boronic Acids
/ chemistry
Carbocyanines
/ chemistry
Cell Line, Tumor
Esters
/ chemistry
Fluorescence
Fluorescent Dyes
/ chemistry
HeLa Cells
Human Umbilical Vein Endothelial Cells
Humans
Hydrogen-Ion Concentration
Lysosomes
/ chemistry
Molecular Probes
/ chemistry
Rhodamines
/ chemistry
Spectroscopy, Near-Infrared
Spironolactone
/ chemistry
Xanthenes
Fluorescence imaging
Fluorescent probes
Lysosomal pH
Near-infrared fluorescence
Rhodamine dyes
Journal
Methods (San Diego, Calif.)
ISSN: 1095-9130
Titre abrégé: Methods
Pays: United States
ID NLM: 9426302
Informations de publication
Date de publication:
15 09 2019
15 09 2019
Historique:
received:
29
03
2019
revised:
11
07
2019
accepted:
12
07
2019
pubmed:
26
7
2019
medline:
9
7
2020
entrez:
26
7
2019
Statut:
ppublish
Résumé
Sterically hindered fluorescent probes (A-C) have been developed by introducing 2-aminophenylboronic acid pinacol ester to a traditional, A, a near-infrared rhodamine dye, B, and a near-infrared hemicyanine dye, C, forming closed spirolactam ring structures. Probe A was non-fluorescent under basic pH conditions whereas probes B and C were moderately fluorescent with fluorescence quantum yields of 9% and 5% in pH 7.4 PBS buffer containing 1% ethanol, respectively. With all probes increasing acidity leads to significant increases in fluorescence at 580 nm, 644 and 744 nm for probes A, B and C with fluorescence quantum yields of 26%, 21% and 10% in pH 4.5 PBS buffer containing 1% ethanol, respectively. Probes A, B and C were calculated to have pK
Identifiants
pubmed: 31344405
pii: S1046-2023(19)30024-6
doi: 10.1016/j.ymeth.2019.07.012
pmc: PMC6851477
mid: NIHMS1535378
pii:
doi:
Substances chimiques
Boronic Acids
0
Carbocyanines
0
Esters
0
Fluorescent Dyes
0
Molecular Probes
0
Rhodamines
0
Xanthenes
0
tetramethylene hemicyanine
0
Spironolactone
27O7W4T232
3-aminobenzeneboronic acid
30418-59-8
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
40-50Subventions
Organisme : NIGMS NIH HHS
ID : R15 GM114751
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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