Potent therapeutic effects of ruscogenin on gastric ulcer established by acetic acid.
Animals
Chronic Disease
Collagen
/ metabolism
Cytokines
/ metabolism
Dinoprostone
/ metabolism
Disease Models, Animal
Epidermal Growth Factor
/ metabolism
Female
Microscopy, Electron, Transmission
Ophiopogon
/ chemistry
Parietal Cells, Gastric
/ pathology
Peroxidases
/ metabolism
Phytotherapy
Rats, Sprague-Dawley
Spirostans
/ administration & dosage
Stomach Ulcer
/ drug therapy
Tumor Necrosis Factor-alpha
/ metabolism
Collagen
Gastric ulcer
Inflammation
Ruscogenin
Ultrastructure
Journal
Asian journal of surgery
ISSN: 0219-3108
Titre abrégé: Asian J Surg
Pays: Netherlands
ID NLM: 8900600
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
22
04
2019
revised:
13
06
2019
accepted:
01
07
2019
pubmed:
28
7
2019
medline:
20
9
2020
entrez:
27
7
2019
Statut:
ppublish
Résumé
The present study investigated the potent therapeutic effects of Ruscogenin, main steroid sapogenin of traditional Chinese plant called 'Ophiopogon japonicas', on chronic ulcer model established with acetic acid in rats. 24 rats were attenuated to the sham (2 ml/kg/day isotonic solution), control (untreated ulcer) and treatment (3 ml/kg/day ruscogenin) groups. After treatment for 2 weeks, gastric tissues were collected and prepared for light microscopic (H&E), immunohistochemical (Collagen I, III and IV) and biochemical analysis [Epidermal growth factor (EGF), Prostaglandin E2 (PGE2), Tumor Necrosis Factor alpha (TNF-α), Interleukin 6 and 8 (IL-6 and IL-8), Lipid Peroxidase (LPO), Myeloperoxidase (MPO), Glutathione (GSH) and Glutathione Peroxidase (GSH-Px)] and transmission electron microscopy (TEM). Macroscopic scoring showed that the ulceration area of ruscogenin-treated group decreased compared with control group. Immunohistochemical analysis revealed ruscogenin ameliorated and restored the levels of Collagen I and IV to the levels of sham group. Tissue levels of EGF and PGE2 enhanced significantly in untreated ulcer group while were higher in treated ulcer group than the control group. TNF-α, IL-6, IL-8, LPO, MPO levels increased significantly in control group whereas decreased in treated rats after ruscogenin treatment. However, levels of GSH and GSH-Px increased significantly in treatment group. TEM showed chief cells and parietal cells of ulcer group having degenerated organelles while ruscogenin group had normal ultrastructure of cells. There are potent anti-inflammatory and anti-oxidant effects of ruscogenin on gastric ulcer and may be successfully used as a safe and therapeutic agent in treatment of peptic ulcer.
Sections du résumé
BACKGROUND/OBJECTIVE
OBJECTIVE
The present study investigated the potent therapeutic effects of Ruscogenin, main steroid sapogenin of traditional Chinese plant called 'Ophiopogon japonicas', on chronic ulcer model established with acetic acid in rats.
METHODS
METHODS
24 rats were attenuated to the sham (2 ml/kg/day isotonic solution), control (untreated ulcer) and treatment (3 ml/kg/day ruscogenin) groups. After treatment for 2 weeks, gastric tissues were collected and prepared for light microscopic (H&E), immunohistochemical (Collagen I, III and IV) and biochemical analysis [Epidermal growth factor (EGF), Prostaglandin E2 (PGE2), Tumor Necrosis Factor alpha (TNF-α), Interleukin 6 and 8 (IL-6 and IL-8), Lipid Peroxidase (LPO), Myeloperoxidase (MPO), Glutathione (GSH) and Glutathione Peroxidase (GSH-Px)] and transmission electron microscopy (TEM).
RESULTS
RESULTS
Macroscopic scoring showed that the ulceration area of ruscogenin-treated group decreased compared with control group. Immunohistochemical analysis revealed ruscogenin ameliorated and restored the levels of Collagen I and IV to the levels of sham group. Tissue levels of EGF and PGE2 enhanced significantly in untreated ulcer group while were higher in treated ulcer group than the control group. TNF-α, IL-6, IL-8, LPO, MPO levels increased significantly in control group whereas decreased in treated rats after ruscogenin treatment. However, levels of GSH and GSH-Px increased significantly in treatment group. TEM showed chief cells and parietal cells of ulcer group having degenerated organelles while ruscogenin group had normal ultrastructure of cells.
CONCLUSION
CONCLUSIONS
There are potent anti-inflammatory and anti-oxidant effects of ruscogenin on gastric ulcer and may be successfully used as a safe and therapeutic agent in treatment of peptic ulcer.
Identifiants
pubmed: 31345657
pii: S1015-9584(19)30365-3
doi: 10.1016/j.asjsur.2019.07.001
pii:
doi:
Substances chimiques
Cytokines
0
Spirostans
0
Tumor Necrosis Factor-alpha
0
Epidermal Growth Factor
62229-50-9
Collagen
9007-34-5
ruscogenin
BXI92R2VUJ
Peroxidases
EC 1.11.1.-
Dinoprostone
K7Q1JQR04M
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
405-416Informations de copyright
Copyright © 2019. Published by Elsevier Taiwan LLC.