TAK1 converts Sequestosome 1/p62 from an autophagy receptor to a signaling platform.
Autophagosomes
/ metabolism
Autophagy
/ genetics
HEK293 Cells
HeLa Cells
Humans
Immunoblotting
Immunoprecipitation
MAP Kinase Kinase Kinases
/ genetics
Microscopy, Confocal
RNA, Small Interfering
/ genetics
RNA-Binding Proteins
/ genetics
Sequestosome-1 Protein
/ genetics
Signal Transduction
/ genetics
HIV-1
TLR3
TRIM5α
anti-inflammatory
tripartite motif
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
06
04
2018
revised:
14
06
2019
accepted:
02
07
2019
pubmed:
28
7
2019
medline:
2
6
2020
entrez:
27
7
2019
Statut:
ppublish
Résumé
The protein p62/Sequestosome 1 (p62) has been described as a selective autophagy receptor and independently as a platform for pro-inflammatory and other intracellular signaling. How these seemingly disparate functional roles of p62 are coordinated has not been resolved. Here, we show that TAK1, a kinase involved in immune signaling, negatively regulates p62 action in autophagy. TAK1 reduces p62 localization to autophagosomes, dampening the autophagic degradation of both p62 and p62-directed autophagy substrates. TAK1 also relocalizes p62 into dynamic cytoplasmic bodies, a phenomenon that accompanies the stabilization of TAK1 complex components. On the other hand, p62 facilitates the assembly and activation of TAK1 complexes, suggesting a connection between p62's signaling functions and p62 body formation. Thus, TAK1 governs p62 action, switching it from an autophagy receptor to a signaling platform. This ability of TAK1 to disable p62 as an autophagy receptor may allow certain autophagic substrates to accumulate when needed for cellular functions.
Identifiants
pubmed: 31347268
doi: 10.15252/embr.201846238
pmc: PMC6726904
doi:
Substances chimiques
P62 protein, human
0
RNA, Small Interfering
0
RNA-Binding Proteins
0
SQSTM1 protein, human
0
Sequestosome-1 Protein
0
MAP Kinase Kinase Kinases
EC 2.7.11.25
MAP kinase kinase kinase 7
EC 2.7.11.25
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e46238Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM121176
Pays : United States
Organisme : HHS | NIH | National Institute of General Medical Sciences (NIGMS)
ID : P20GM121176
Pays : International
Organisme : HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID)
ID : R21AI131964
Pays : International
Informations de copyright
© 2019 The Authors.
Références
Mol Cell. 2014 Jan 23;53(2):167-78
pubmed: 24462201
Curr Opin Cell Biol. 2010 Apr;22(2):124-31
pubmed: 20034776
Immunity. 2012 Aug 24;37(2):223-34
pubmed: 22921120
J Biol Chem. 2010 Jul 16;285(29):22576-91
pubmed: 20452972
Cell Death Differ. 2015 Mar;22(3):377-88
pubmed: 25257172
Cell Rep. 2015 Aug 25;12(8):1339-52
pubmed: 26279575
Nature. 2002 May 9;417(6885):177-82
pubmed: 12000969
Cell Cycle. 2013 Jun 15;12(12):1868-78
pubmed: 23708522
Sci Signal. 2018 Dec 04;11(559):
pubmed: 30514811
Cell Res. 2018 Apr;28(4):405-415
pubmed: 29507397
Cell. 2016 May 19;165(5):1055-1066
pubmed: 27203110
J Biochem. 2007 Feb;141(2):137-45
pubmed: 17190786
Nat Cell Biol. 2013 Apr;15(4):406-16
pubmed: 23524951
Sci Signal. 2010 May 25;3(123):ra42
pubmed: 20501938
Mol Cell. 2011 Oct 7;44(1):134-46
pubmed: 21981924
J Cell Biol. 2004 Mar 1;164(5):667-75
pubmed: 14981091
Science. 2005 Mar 11;307(5715):1621-5
pubmed: 15761153
Nat Cell Biol. 2014 Jun;16(6):495-501
pubmed: 24875736
FEBS J. 2015 Dec;282(24):4672-8
pubmed: 26432171
Cell Rep. 2017 Apr 4;19(1):188-202
pubmed: 28380357
Nat Cell Biol. 2010 Mar;12(3):213-23
pubmed: 20173742
J Immunol. 2009 Nov 1;183(9):5909-16
pubmed: 19812211
EMBO J. 2016 Aug 15;35(16):1779-92
pubmed: 27370208
J Cell Biol. 1997 Dec 29;139(7):1687-95
pubmed: 9412464
J Virol. 2010 Jun;84(12):5997-6006
pubmed: 20357094
Cancer Cell. 2008 Apr;13(4):343-54
pubmed: 18394557
Cell. 2016 Oct 20;167(3):606-609
pubmed: 27768885
J Biol Chem. 2003 Sep 5;278(36):34568-81
pubmed: 12813044
J Cell Biol. 2011 Jan 10;192(1):17-27
pubmed: 21220506
J Biol Chem. 2014 Apr 18;289(16):11162-74
pubmed: 24619419
Virology. 2011 Jan 5;409(1):113-20
pubmed: 21035162
EMBO J. 2013 Aug 28;32(17):2336-47
pubmed: 23921551
Dev Cell. 2016 May 23;37(4):337-349
pubmed: 27219062
Cell. 2011 Nov 11;147(4):728-41
pubmed: 22078875
Mol Cell. 2016 Mar 3;61(5):720-733
pubmed: 26942676
Sci Signal. 2014 May 13;7(325):ra45
pubmed: 24825920
Mol Cell. 2016 Mar 3;61(5):654-666
pubmed: 26942670
Nat Rev Immunol. 2013 Oct;13(10):722-37
pubmed: 24064518
Cell Cycle. 2006 Dec;5(24):2852-5
pubmed: 17218788
Nature. 2001 Jul 19;412(6844):346-51
pubmed: 11460167
EMBO J. 2000 Apr 3;19(7):1576-86
pubmed: 10747026
Mol Cell. 2013 Aug 8;51(3):283-96
pubmed: 23911927
Proc Natl Acad Sci U S A. 2016 Apr 12;113(15):4039-44
pubmed: 27035970
Dev Cell. 2014 Aug 25;30(4):394-409
pubmed: 25127057
J Cell Sci. 2016 Mar 1;129(5):881-91
pubmed: 26906420
Nat Genet. 2004 Jun;36(6):585-95
pubmed: 15146184
J Biol Chem. 2007 Aug 17;282(33):24131-45
pubmed: 17580304
Cell Death Differ. 2009 Nov;16(11):1426-37
pubmed: 19713972
PLoS Genet. 2015 Feb 27;11(2):e1004987
pubmed: 25723488
J Cell Biol. 2005 Nov 21;171(4):603-14
pubmed: 16286508
J Cell Sci. 2018 Jul 26;131(14):
pubmed: 29930081
J Cell Sci. 2016 Oct 1;129(19):3562-3573
pubmed: 27562068
Cell. 2009 May 15;137(4):721-35
pubmed: 19427028
EMBO J. 2009 Mar 18;28(6):677-85
pubmed: 19197243
Nat Rev Immunol. 2011 Aug 25;11(9):617-25
pubmed: 21866173
EMBO J. 2011 Nov 11;30(24):4908-20
pubmed: 22081109
Nature. 2004 Feb 26;427(6977):848-53
pubmed: 14985764
Nat Cell Biol. 2017 Oct;19(10):1202-1213
pubmed: 28846094
Immunity. 2007 Jul;27(1):135-44
pubmed: 17658277
Nature. 2011 Apr 21;472(7343):361-5
pubmed: 21512573
Autophagy. 2010 Apr;6(3):330-44
pubmed: 20168092
EMBO J. 2018 Mar 1;37(5):
pubmed: 29343546
Antioxid Redox Signal. 2016 Dec 10;25(17):953-964
pubmed: 27245349
Nat Immunol. 2009 Nov;10(11):1215-21
pubmed: 19820708
J Biochem. 2012 Feb;151(2):157-66
pubmed: 21976705
Nat Commun. 2015 Oct 27;6:8620
pubmed: 26503418
J Proteome Res. 2015 May 1;14(5):2298-311
pubmed: 25815641
J Biol Chem. 2003 May 9;278(19):16713-9
pubmed: 12609980