One piece biopsy for both rapid urease test and cultivation of Helicobacter pylori.
Adolescent
Adult
Aged
Aged, 80 and over
Biopsy
/ methods
Child
Child, Preschool
Diagnostic Tests, Routine
/ methods
Female
Gastric Mucosa
/ microbiology
Helicobacter Infections
/ diagnosis
Helicobacter pylori
/ growth & development
Humans
Infant
Male
Middle Aged
Polymerase Chain Reaction
Sensitivity and Specificity
Stomach
/ microbiology
Stomach Diseases
/ diagnosis
Urease
/ analysis
Young Adult
Biopsy specimens
Gastric disorders
Helicobacter pylori
Rapid urease test
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
07
04
2019
revised:
22
07
2019
accepted:
22
07
2019
pubmed:
28
7
2019
medline:
23
6
2020
entrez:
27
7
2019
Statut:
ppublish
Résumé
Increasing antibiotic resistance among Helicobacter pylori isolates and the unsuccessful attempts at eradication can impose many costs to both healthcare systems and patients. The present study intended to find a way from which H. pylori could be isolated from biopsies with less invasive procedures undertaken on infected patients. A biopsy specimen from each patient with gastric disorders was put into urea-containing agar. After two hours, the specimens were removed from agar and placed into tubes containing 1 ml 20% glucose solution. Then, the specimens were inoculated onto the Columbia agar and incubated under microaerophilic conditions. The grown colonies were identified as H.pylori based on the microbiology tests and PCR. Overall, 449 biopsy specimens were collected from the patients. Of all biopsies, 219 (48.8%) revealed positive results in the rapid urease test. Using the aforementioned method, 158 (35.2%) culture positive biopsy specimens were obtained. The researchers attempted to use one biopsy specimen for both rapid urease and culture tests. This method causes fewer injuries of gastric tissue and allows antimicrobial susceptibility testing and characterization in detail of the isolated organism.
Sections du résumé
BACKGROUND & AIMS
Increasing antibiotic resistance among Helicobacter pylori isolates and the unsuccessful attempts at eradication can impose many costs to both healthcare systems and patients. The present study intended to find a way from which H. pylori could be isolated from biopsies with less invasive procedures undertaken on infected patients.
METHODS
A biopsy specimen from each patient with gastric disorders was put into urea-containing agar. After two hours, the specimens were removed from agar and placed into tubes containing 1 ml 20% glucose solution. Then, the specimens were inoculated onto the Columbia agar and incubated under microaerophilic conditions. The grown colonies were identified as H.pylori based on the microbiology tests and PCR.
RESULTS
Overall, 449 biopsy specimens were collected from the patients. Of all biopsies, 219 (48.8%) revealed positive results in the rapid urease test. Using the aforementioned method, 158 (35.2%) culture positive biopsy specimens were obtained.
CONCLUSION
The researchers attempted to use one biopsy specimen for both rapid urease and culture tests. This method causes fewer injuries of gastric tissue and allows antimicrobial susceptibility testing and characterization in detail of the isolated organism.
Identifiants
pubmed: 31348953
pii: S0167-7012(19)30290-8
doi: 10.1016/j.mimet.2019.105674
pii:
doi:
Substances chimiques
Urease
EC 3.5.1.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105674Informations de copyright
Copyright © 2019. Published by Elsevier B.V.