Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement.
Journal
Nature structural & molecular biology
ISSN: 1545-9985
Titre abrégé: Nat Struct Mol Biol
Pays: United States
ID NLM: 101186374
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
18
04
2019
accepted:
17
06
2019
pubmed:
31
7
2019
medline:
20
2
2020
entrez:
31
7
2019
Statut:
ppublish
Résumé
Many stem cells undergo asymmetric division to produce a self-renewing stem cell and a differentiating daughter cell. Here we show that, similarly to H3, histone H4 is inherited asymmetrically in Drosophila melanogaster male germline stem cells undergoing asymmetric division. In contrast, both H2A and H2B are inherited symmetrically. By combining super-resolution microscopy and chromatin fiber analyses with proximity ligation assays on intact nuclei, we find that old H3 is preferentially incorporated by the leading strand, whereas newly synthesized H3 is enriched on the lagging strand. Using a sequential nucleoside analog incorporation assay, we detect a high incidence of unidirectional replication fork movement in testes-derived chromatin and DNA fibers. Biased fork movement coupled with a strand preference in histone incorporation would explain how asymmetric old and new H3 and H4 are established during replication. These results suggest a role for DNA replication in patterning epigenetic information in asymmetrically dividing cells in multicellular organisms.
Identifiants
pubmed: 31358945
doi: 10.1038/s41594-019-0269-z
pii: 10.1038/s41594-019-0269-z
pmc: PMC6684448
mid: NIHMS1532202
doi:
Substances chimiques
Drosophila Proteins
0
Histones
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
732-743Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM007231
Pays : United States
Organisme : NIGMS NIH HHS
ID : F31 GM115149
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM112008
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM127075
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM033397
Pays : United States
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