Antibodies against neural antigens in patients with acute stroke: joint results of three independent cohort studies.

Anti-CASPR2 antibodies Anti-GAD65 antibodies Anti-NMDAR antibodies Anti-aquaporin antibodies Antineural antibodies Stroke

Journal

Journal of neurology
ISSN: 1432-1459
Titre abrégé: J Neurol
Pays: Germany
ID NLM: 0423161

Informations de publication

Date de publication:
Nov 2019
Historique:
received: 02 05 2019
accepted: 11 07 2019
revised: 10 07 2019
pubmed: 31 7 2019
medline: 21 3 2020
entrez: 31 7 2019
Statut: ppublish

Résumé

Ischemic stroke (IS) and hemorrhagic stroke (HemS) typically lead to a breakdown of the blood-brain barrier with neural antigen presentation. This presentation could potentially generate destructive auto-immune responses. Pre-existing antineuronal and antiglial antibodies (AA), predominantly NMDA receptor antibodies, have been reported in patients with stroke. This article summarizes three independent prospective studies, the Lübeck cohort (LC), Barcelona cohort (BC), and Heidelberg cohort (HC), exploring the frequency and clinical relevance of AA in patients with acute stroke (AS). In all cohorts together, 344 consecutive patients admitted with AS (322 × IS, 22 × HemS) were screened for AA in serum at admission. Clinical outcome parameters as well as a second AA screening were available at 30 days in the LC or at 90 days in the BC. A control group was included in the BC (20 subjects free from neurological disease) and the HC (78 neurological and ophthalmological patients without evidence for stroke). The rate of positivity for AA was similar in control subjects and AS patients (13%, 95% CI [7%, 22%] vs. 13%, 95% CI [10%, 17%]; p = 0.46) with no significant difference between cohorts (LC 25/171, BC 12/75, HC 9/98). No patient had developed new AA after 30 days, whereas 2 out of 60 patients had developed new AA after 90 days. AA positive patients did not exhibit significant differences to AA negative patients in stroke subtype (LC, BC), initial stroke severity (BC, LC, HC), infarct volume (BC), and functional status at admission (BC, LC, HC) and follow-up (BC, LC). AS does not induce AA to a relevant degree. Pre-existing AA can be found in the serum of stroke patients, but they do not have a significant association with clinical features and outcomes.

Sections du résumé

BACKGROUND AND PURPOSE OBJECTIVE
Ischemic stroke (IS) and hemorrhagic stroke (HemS) typically lead to a breakdown of the blood-brain barrier with neural antigen presentation. This presentation could potentially generate destructive auto-immune responses. Pre-existing antineuronal and antiglial antibodies (AA), predominantly NMDA receptor antibodies, have been reported in patients with stroke. This article summarizes three independent prospective studies, the Lübeck cohort (LC), Barcelona cohort (BC), and Heidelberg cohort (HC), exploring the frequency and clinical relevance of AA in patients with acute stroke (AS).
METHODS METHODS
In all cohorts together, 344 consecutive patients admitted with AS (322 × IS, 22 × HemS) were screened for AA in serum at admission. Clinical outcome parameters as well as a second AA screening were available at 30 days in the LC or at 90 days in the BC. A control group was included in the BC (20 subjects free from neurological disease) and the HC (78 neurological and ophthalmological patients without evidence for stroke).
RESULTS RESULTS
The rate of positivity for AA was similar in control subjects and AS patients (13%, 95% CI [7%, 22%] vs. 13%, 95% CI [10%, 17%]; p = 0.46) with no significant difference between cohorts (LC 25/171, BC 12/75, HC 9/98). No patient had developed new AA after 30 days, whereas 2 out of 60 patients had developed new AA after 90 days. AA positive patients did not exhibit significant differences to AA negative patients in stroke subtype (LC, BC), initial stroke severity (BC, LC, HC), infarct volume (BC), and functional status at admission (BC, LC, HC) and follow-up (BC, LC).
CONCLUSIONS CONCLUSIONS
AS does not induce AA to a relevant degree. Pre-existing AA can be found in the serum of stroke patients, but they do not have a significant association with clinical features and outcomes.

Identifiants

pubmed: 31359201
doi: 10.1007/s00415-019-09470-2
pii: 10.1007/s00415-019-09470-2
doi:

Substances chimiques

Autoantibodies 0
Autoantigens 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2772-2779

Subventions

Organisme : European Union Framework Program FP7
ID : 607962
Organisme : ISCIII-Subdireccion General de Evaluacion
ID : FIS P115/00430

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Auteurs

Georg Royl (G)

Department of Neurology, University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany. georg.royl@neuro.uni-luebeck.de.
Center of Brain, Behavior and Metabolism, University of Lübeck, Lübeck, Germany. georg.royl@neuro.uni-luebeck.de.

Tsafack Judicael Fokou (TJ)

Department of Neurology, University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.

Rittika Chunder (R)

Euroimmun Medizinische Labordiagnostika, Lübeck, Germany.

Rakad Isa (R)

Department of Neurology, University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.

Thomas F Münte (TF)

Department of Neurology, University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.
Center of Brain, Behavior and Metabolism, University of Lübeck, Lübeck, Germany.

Klaus-Peter Wandinger (KP)

Department of Neurology, University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.
Institute of Clinical Chemistry, University Hospital of Schleswig-Holstein, Holstein, Germany.

Markus Schwaninger (M)

Center of Brain, Behavior and Metabolism, University of Lübeck, Lübeck, Germany.
Institute of Experimental and Clinical Pharmacology and Toxicology, University of Lübeck, Lübeck, Germany.
Department of Neurology, University of Heidelberg, Heidelberg, Germany.

Oliver Herrmann (O)

Department of Neurology, University of Heidelberg, Heidelberg, Germany.

José Manuel Valdueza (JM)

Neurological Center, Segeberger Kliniken, Bad Segeberg, Germany.

Jan Brocke (J)

Neurological Center, Segeberger Kliniken, Bad Segeberg, Germany.

Martin Willkomm (M)

Geriatric Research Group, Krankenhaus Rotes Kreuz, Lübeck, Germany.

Dietrich Willemsen (D)

SANA Kliniken Ostholstein, Middelburg, Germany.

Gerd U Auffarth (GU)

Department of Ophthalmology, University of Heidelberg, Heidelberg, Germany.

Swantje Mindorf (S)

Euroimmun Medizinische Labordiagnostika, Lübeck, Germany.

Britta Brix (B)

Euroimmun Medizinische Labordiagnostika, Lübeck, Germany.

Angel Chamorro (A)

Department of Neuroscience, Comprehensive Stroke Center, Hospital Clinic, Barcelona, Spain.
August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain.

Anna Planas (A)

August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain.
Department of Brain Ischemia and Neurodegeneration, Institut d'Investigacions Biomèdiques de Barcelona, Consejo Superior de Investigaciones Científicas, Barcelona, Spain.

Xabier Urra (X)

Department of Neuroscience, Comprehensive Stroke Center, Hospital Clinic, Barcelona, Spain.
August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain.

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