Identification of Antigens in Immune Complexes.

Antibodies / analysis Antigens / analysis Chromatography, Liquid / methods Papain / analysis Tandem Mass Spectrometry / methods
Antibody Antigen Immune complex Nano-LC-MS/MS Papain

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 1 8 2019
pubmed: 1 8 2019
medline: 31 3 2020
Statut: ppublish

Résumé

Comprehensive identification and profiling of antigens in immune complexes (IC-antigens) is useful to provide insights into pathophysiology and could form the basis for novel diagnostic and treatment strategies for many immune-related diseases. Immune complexome analysis is the method for comprehensively identifying and profiling IC-antigens in biological fluids (such as serum and cerebrospinal fluid). Here, we describe an IC-antigen detection method; specifically, ICs in biological fluids are captured by using protein G- or protein A-coated beads, are subjected to papain-digestion, elution, and tryptic digestion, and are analyzed by nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS).

Identifiants

DOI: 10.1007/978-1-4939-9597-4_22 PMID: 31364061
pubmed: 31364061
doi: 10.1007/978-1-4939-9597-4_22
doi:

Substances chimiques

Antibodies 0
Antigens 0
Papain EC 3.4.22.2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

333-338

Auteurs

Nozomi Aibara (N)

Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.

Kaname Ohyama (K)

Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan. k-ohyama@nagasaki-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Identification of Antigens in Immune Complexes.
questionsmedicales.fr › Facteurs biologiques › Antigènes › Identification of Antigens in Immune Complexes.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Identification of Antigens in Immune Complexes.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Endopeptidases › Cysteine endopeptidases › Papaïne › Identification of Antigens in Immune Complexes.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Identification of Antigens in Immune Complexes.