Characteristic Morphological Changes and Rapid Actin Accumulation in Serum-MAF-treated Macrophages.
THP-1
actin accumulation
morphological change
serum-MAF (serum-derived macrophage activating factor)
time-lapse image
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
23
05
2019
revised:
23
06
2019
accepted:
24
06
2019
entrez:
2
8
2019
pubmed:
2
8
2019
medline:
7
8
2019
Statut:
ppublish
Résumé
Serum-derived macrophage activating factor, serum-MAF, is known to increase the phagocytic activity of macrophages by enhancing the engulfment efficiency. To elucidate the mechanisms underlying phagocytic activation, morphological changes were observed and analyzed. Morphological changes in macrophages were observed and quantitatively analyzed using scanning electron microscope (SEM) and confocal microscope. SEM and confocal microscopy images revealed frill-like structures and active actin accumulations, respectively, in serum-MAF treated macrophages. Actin accumulation was induced within 5 min following serum-MAF treatment. Serum-MAF induced a rapid rearrangement of cytoskeletal actin and enhanced phagocytic activity. Findings of the current study may contribute to the development of techniques that facilitate activation of the human immune system, which in turn may be beneficial for cancer immunotherapy.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Serum-derived macrophage activating factor, serum-MAF, is known to increase the phagocytic activity of macrophages by enhancing the engulfment efficiency. To elucidate the mechanisms underlying phagocytic activation, morphological changes were observed and analyzed.
MATERIALS AND METHODS
METHODS
Morphological changes in macrophages were observed and quantitatively analyzed using scanning electron microscope (SEM) and confocal microscope.
RESULTS
RESULTS
SEM and confocal microscopy images revealed frill-like structures and active actin accumulations, respectively, in serum-MAF treated macrophages. Actin accumulation was induced within 5 min following serum-MAF treatment.
CONCLUSION
CONCLUSIONS
Serum-MAF induced a rapid rearrangement of cytoskeletal actin and enhanced phagocytic activity. Findings of the current study may contribute to the development of techniques that facilitate activation of the human immune system, which in turn may be beneficial for cancer immunotherapy.
Identifiants
pubmed: 31366556
pii: 39/8/4533
doi: 10.21873/anticanres.13630
doi:
Substances chimiques
Actins
0
MAF protein, human
0
Proto-Oncogene Proteins c-maf
0
Vitamin D-Binding Protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4533-4537Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.