The lipidome of primary murine white, brite, and brown adipocytes-Impact of beta-adrenergic stimulation.
Adipocytes, Brown
/ metabolism
Adipocytes, White
/ metabolism
Adipose Tissue, Beige
/ metabolism
Adipose Tissue, Brown
/ metabolism
Adipose Tissue, White
/ metabolism
Adrenergic Agents
Animals
Cell Differentiation
Lipid Metabolism
/ physiology
Lipidomics
/ methods
Lipids
/ physiology
Male
Mice
Mice, 129 Strain
Mice, Knockout
Receptors, Adrenergic, beta
/ metabolism
Signal Transduction
Uncoupling Protein 1
/ genetics
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
18
02
2019
accepted:
15
07
2019
revised:
13
08
2019
pubmed:
2
8
2019
medline:
28
2
2020
entrez:
2
8
2019
Statut:
epublish
Résumé
Lipid species patterns are conserved within cells to maintain physicochemical properties of membranes and cellular functions. We present the lipidome, including sterols, glycerolipids (GLs), glycerophospholipids (GPLs), and sphingolipids (SLs), of primary ex vivo differentiated (I) white, (II) brite, and (III) brown adipocytes derived from primary preadipocytes isolated from (I) epididymal white, (II) inguinal white, and (III) intrascapular brown adipose tissue. Quantitative lipidomics revealed significantly decreased fractions of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), with longer (C > 36) and more polyunsaturated species, as well as lower levels of cardiolipin (CL) in white than in brite and brown adipocytes. Together, the brite and brown lipidome was comparable and indicates differences in membrane lipid packing density compared with white adipocytes. Changes in ceramide species profile could be related to the degree of browning. Beta-adrenergic stimulation of brown adipocytes led to generation of saturated lyso-PC (LPC) increasing uncoupling protein (UCP) 1-mediated leak respiration. Application of stable isotope labeling showed that LPC formation was balanced by an increased de novo synthesis of PC.
Identifiants
pubmed: 31369546
doi: 10.1371/journal.pbio.3000412
pii: PBIOLOGY-D-19-00447
pmc: PMC6692052
doi:
Substances chimiques
Adrenergic Agents
0
Lipids
0
Receptors, Adrenergic, beta
0
Uncoupling Protein 1
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3000412Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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