Ligation and Ligases.

Bacteriophage T4 / enzymology DNA / metabolism DNA Ligases / metabolism Enzyme Stability Escherichia coli / enzymology RNA / metabolism Temperature

Journal

Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530

Informations de publication

Date de publication:
01 08 2019
Historique:
entrez: 3 8 2019
pubmed: 3 8 2019
medline: 29 8 2020
Statut: epublish

Résumé

DNA ligases are used chiefly to create novel combinations of nucleic acid molecules and to attach them to vectors before molecular cloning. They are either of bacterial origin or bacteriophage encoded and have different properties, as discussed here.

Identifiants

DOI: 10.1101/pdb.top101352 PMID: 31371476
pubmed: 31371476
pii: 2019/8/pdb.top101352
doi: 10.1101/pdb.top101352
doi:

Substances chimiques

RNA 63231-63-0
DNA 9007-49-2
DNA Ligases EC 6.5.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2019 Cold Spring Harbor Laboratory Press.

Auteurs

Michael R Green (MR)

Joseph Sambrook (J)

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Classifications MeSH

questionsmedicales.fr › Virus › Virus à ADN › Caudovirales › Myoviridae › Bactériophage T4 › Ligation and Ligases.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Ligation and Ligases.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Ligases › Polynucleotide ligases › DNA ligases › Ligation and Ligases.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Ligation and Ligases.
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Ligation and Ligases.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › Ligation and Ligases.
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Ligation and Ligases.