Whole genome sequencing revealed new molecular characteristics in multidrug resistant staphylococci recovered from high frequency touched surfaces in London.
Anti-Bacterial Agents
/ therapeutic use
Bacterial Proteins
/ genetics
Drug Resistance, Multiple, Bacterial
/ genetics
Hospitals
Humans
London
/ epidemiology
Microbial Sensitivity Tests
Polymerase Chain Reaction
Residence Characteristics
Staphylococcal Infections
/ drug therapy
Staphylococcus epidermidis
/ chemistry
Surface Properties
Touch
Whole Genome Sequencing
/ methods
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
01 08 2019
01 08 2019
Historique:
received:
02
01
2019
accepted:
13
06
2019
entrez:
3
8
2019
pubmed:
3
8
2019
medline:
22
9
2020
Statut:
epublish
Résumé
The rise of antibiotic resistance (AMR) is one of the most important public health threats worldwide.Today, increasing attention is being paid to multidrug resistant staphylococci isolated from healthcare and non-healthcare environments as the treatment of these bacteria has become increasingly difficult. In this study, we compared staphylococci isolates recovered from high frequency touched surfaces from public areas in the community and hospitals in East and West London. 281 out of 600 (46.83%) staphylococci isolates recovered were multidrug resistant, of which 49 (8.17%) were mecA positive. There was significantly higher proportion of multidrug resistant staphylococci (P = 0.0002) in East London (56.7%) compared to West London (49.96%). The most common species identified as multidrug resistant were S. epidermidis, S. haemolyticus and S. hominis, whereas penicillin, fusidic acid and erythromycin were the most frequent antibiotics the isolates were resistant to. Whole genome sequenced of mecA positive isolates revealed that S. sciuri isolates carried the mecA1 gene, which has only 84.43% homology with mecA. In addition, other frequently identified resistance genes included blaZ, qacA/B and dfrC. We have also identified a diverse range of SCCmec types, many of which were untypable due to carrying a novel combination of ccr genes or multiple ccr complexes.
Identifiants
pubmed: 31371820
doi: 10.1038/s41598-019-45886-6
pii: 10.1038/s41598-019-45886-6
pmc: PMC6675788
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
MecA protein, Staphylococcus epidermidis
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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