Characteristics of a recombinant Lentinula edodes endoglucanase and its potential for application in silage of rape straw.

Biotechnology Brassica rapa / chemistry Cellulase / chemistry Fermentation Hydrolysis Models, Molecular Mutagenesis, Site-Directed Protein Conformation Recombinant Proteins / chemistry Shiitake Mushrooms / enzymology
Ensiling Rape straw Recombinant Lentinula edodes endoglucanase Saccharification efficiency

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 Oct 2019
Historique:
received: 20 06 2019
revised: 29 07 2019
accepted: 29 07 2019
pubmed: 3 8 2019
medline: 14 4 2020
entrez: 3 8 2019
Statut: ppublish

Résumé

An experiment was conducted to determine the characteristics of recombinant endoglucanase and its effects on rape straw silage. The endoglucanase from Lentinula edodes (LeCel12A) was produced in Pichia pastoris and shown maximum activity at 40 °C and pH 3.0. The LeCel12A exhibited preferential hydrolysis of carboxymethylcellulose. The activity of LeCel12A could be enhanced by MnCl

Identifiants

DOI: 10.1016/j.ijbiomac.2019.07.199 PMID: 31374269
pubmed: 31374269
pii: S0141-8130(19)34642-2
doi: 10.1016/j.ijbiomac.2019.07.199
pii:
doi:

Substances chimiques

Recombinant Proteins 0
Cellulase EC 3.2.1.4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

49-56

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Lizhi Li (L)

Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; College of Life science and Resources and Environment, Yichun University, Yichun, 336000, China.

Chanjuan Liu (C)

Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.

Mingren Qu (M)

Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.

Wenjing Zhang (W)

Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.

Ke Pan (K)

Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.

Kehui OuYang (K)

Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.

Xiaozhen Song (X)

Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.

Xianghui Zhao (X)

Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China. Electronic address: zhaoxh001@163.com.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Biotechnologie › Characteristics of a recombinant Lentinula...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Brassica › Brassica rapa › Characteristics of a recombinant Lentinula...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Glucosidases › Cellulases › Cellulase › Characteristics of a recombinant Lentinula...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Fermentation › Characteristics of a recombinant Lentinula...
questionsmedicales.fr › Phénomènes chimiques › Hydrolyse › Characteristics of a recombinant Lentinula...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Characteristics of a recombinant Lentinula...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Mutagenèse dirigée › Characteristics of a recombinant Lentinula...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Characteristics of a recombinant Lentinula...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Characteristics of a recombinant Lentinula...
questionsmedicales.fr › Eucaryotes › Champignons › Basidiomycota › Agaricales › Lentinula › Champignons shiitake › Characteristics of a recombinant Lentinula...