Molecular codes and in vitro generation of hypocretin and melanin concentrating hormone neurons.


Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
20 08 2019
Historique:
pubmed: 4 8 2019
medline: 24 3 2020
entrez: 4 8 2019
Statut: ppublish

Résumé

Hypocretin/orexin (HCRT) and melanin concentrating hormone (MCH) neuropeptides are exclusively produced by the lateral hypothalamus and play important roles in sleep, metabolism, reward, and motivation. Loss of HCRT (ligands or receptors) causes the sleep disorder narcolepsy with cataplexy in humans and in animal models. How these neuropeptides are produced and involved in diverse functions remain unknown. Here, we developed methods to sort and purify HCRT and MCH neurons from the mouse late embryonic hypothalamus. RNA sequencing revealed key factors of fate determination for HCRT (

Identifiants

pubmed: 31375626
pii: 1902148116
doi: 10.1073/pnas.1902148116
pmc: PMC6708384
doi:

Substances chimiques

Hcrt protein, mouse 0
Hypothalamic Hormones 0
Intercellular Signaling Peptides and Proteins 0
Melanins 0
Nerve Tissue Proteins 0
Orexins 0
Pituitary Hormones 0
QRFP peptide 0
melanin-concentrating hormone 67382-96-1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

17061-17070

Informations de copyright

Copyright © 2019 the Author(s). Published by PNAS.

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

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Auteurs

Ali Seifinejad (A)

Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, 1005 Lausanne, Switzerland; Ali.Seifinejad@unil.ch Mehdi.Tafti@unil.ch.
Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, 1015 Lausanne, Switzerland.

Sha Li (S)

Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, 1005 Lausanne, Switzerland.

Cyril Mikhail (C)

Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, 1015 Lausanne, Switzerland.

Anne Vassalli (A)

Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, 1005 Lausanne, Switzerland.

Sylvain Pradervand (S)

Genomic Technologies Facility, Faculty of Biology and Medicine, University of Lausanne, 1015 Lausanne, Switzerland.

Yoan Arribat (Y)

Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, 1005 Lausanne, Switzerland.

Hassan Pezeshgi Modarres (H)

Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

Bridget Allen (B)

Biomedicine Division, Cardiff School of Biosciences, Cardiff University, CF10 3AX Cardiff, United Kingdom.

Rosalind M John (RM)

Biomedicine Division, Cardiff School of Biosciences, Cardiff University, CF10 3AX Cardiff, United Kingdom.

Francesca Amati (F)

Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, 1005 Lausanne, Switzerland.

Mehdi Tafti (M)

Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, 1005 Lausanne, Switzerland; Ali.Seifinejad@unil.ch Mehdi.Tafti@unil.ch.

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