Polyomaviruses shedding in stool of patients with hematological disorders: detection analysis and study of the non-coding control region's genetic variability.
Gastrointestinal tract
HPyVs
Hematological patients
NCCR/VP1
Phylogenetic analysis
Stool samples
Journal
Medical microbiology and immunology
ISSN: 1432-1831
Titre abrégé: Med Microbiol Immunol
Pays: Germany
ID NLM: 0314524
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
11
04
2019
accepted:
25
07
2019
pubmed:
4
8
2019
medline:
7
3
2020
entrez:
4
8
2019
Statut:
ppublish
Résumé
Fragmented data are available on the human polyomaviruses (HPyVs) prevalence in the gastrointestinal tract. Rearrangements in the non-coding control region (NCCR) of JCPyV and BKPyV have been extensively studied and correlated to clinical outcome; instead, little information is available for KIPyV, WUPyV and MCPyV NCCRs. To get insights into the role of HPyVs in the gastrointestinal tract, we investigated JCPyV, BKPyV, KIPyV, WUPyV and MCPyV distribution among hematological patients in concomitance with gastrointestinal symptoms. In addition, NCCRs and VP1 sequences were examined to characterize the strains circulating among the enrolled patients. DNA was extracted from 62 stool samples and qPCR was carried out to detect and quantify JCPyV, BKPyV, KIPyV, WUPyV and MCPyV genomes. Positive samples were subsequently amplified and sequenced for NCCR and VP1 regions. A phylogenetic tree was constructed aligning the obtained VP1 sequences to a set of reference sequences. qPCR revealed low viral loads for all HPyVs searched. Mono and co-infections were detected. A significant correlation was found between gastrointestinal complications and KIPyV infection. Archetype-like NCCRs were found for JCPyV and BKPyV, and a high degree of NCCRs stability was observed for KIPyV, WUPyV and MCPyV. Analysis of the VP1 sequences revealed a 99% identity with the VP1 reference sequences. The study adds important information on HPyVs prevalence and persistence in the gastrointestinal tract. Gastrointestinal signs were correlated with the presence of KIPyV, although definitive conclusions cannot be drawn. HPyVs NCCRs showed a high degree of sequence stability, suggesting that sequence rearrangements are rare in this anatomical site.
Identifiants
pubmed: 31375897
doi: 10.1007/s00430-019-00630-9
pii: 10.1007/s00430-019-00630-9
pmc: PMC6817764
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
845-854Références
J Virol. 2010 Oct;84(20):10448-56
pubmed: 20686041
J Gen Virol. 2008 Aug;89(Pt 8):1849-56
pubmed: 18632955
PLoS One. 2018 Oct 23;13(10):e0206273
pubmed: 30352098
J Infect Dis. 2017 Mar 15;215(6):889-895
pubmed: 28453853
J Virol. 2009 Nov;83(21):10846-56
pubmed: 19640999
J Clin Virol. 2009 Sep;46(1):75-9
pubmed: 19576844
Nat Rev Microbiol. 2013 Apr;11(4):264-76
pubmed: 23474680
Cancer Genomics Proteomics. 2015 Jul-Aug;12(4):189-200
pubmed: 26136219
Clin Microbiol Rev. 2012 Jul;25(3):471-506
pubmed: 22763635
Emerg Infect Dis. 2009 Jan;15(1):134-5
pubmed: 19116080
J Clin Virol. 2009 Jul;45(3):249-54
pubmed: 19515607
Appl Environ Microbiol. 2000 Jan;66(1):238-45
pubmed: 10618230
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14542-6
pubmed: 9405649
Science. 2008 Feb 22;319(5866):1096-100
pubmed: 18202256
Bone Marrow Transplant. 2009 Jan;43(1):43-7
pubmed: 18836489
J Clin Virol. 2009 Mar;44(3):230-4
pubmed: 19171499
J Virol. 2007 Apr;81(8):4130-6
pubmed: 17287263
Bone Marrow Transplant. 2014 May;49(5):664-70
pubmed: 24488049
J Virol. 1990 Jun;64(6):3139-43
pubmed: 2159570
Nature. 1986 Nov 13-19;324(6093):163-6
pubmed: 3785382
J Med Virol. 2009 Sep;81(9):1668-73
pubmed: 19626610
J Infect Dis. 2018 Apr 23;217(10):1601-1611
pubmed: 29409030
J Clin Virol. 2007 Sep;40(1):9-14
pubmed: 17714984
J Clin Microbiol. 2009 Aug;47(8):2388-91
pubmed: 19494079
Lancet. 1971 Jun 19;1(7712):1257-60
pubmed: 4104715
Arch Virol. 2017 Dec;162(12):3745-3752
pubmed: 28884263
Virology. 2005 Jan 20;331(2):209-31
pubmed: 15629766
Virology. 1991 Feb;180(2):553-60
pubmed: 1846488
J Pediatric Infect Dis Soc. 2016 Jun;5(2):210-3
pubmed: 27199472
Virus Res. 2010 May;149(2):190-6
pubmed: 20138933
J Gen Virol. 2016 Jul;97(7):1647-57
pubmed: 27031170
J Clin Microbiol. 2013 Sep;51(9):3107-9
pubmed: 23824769
J Infect Dis. 1993 Jan;167(1):13-20
pubmed: 8380288
Southeast Asian J Trop Med Public Health. 2016 Jan;47(1):23-30
pubmed: 27086422
New Microbiol. 2018 Jul;41(3):179-186
pubmed: 29620790
Appl Environ Microbiol. 2006 Dec;72(12):7894-6
pubmed: 17028225
Nephrol Dial Transplant. 2008 Oct;23(10):3333-6
pubmed: 18503098
J Gen Virol. 2001 May;82(Pt 5):1221-331
pubmed: 11297697
J Infect Dis. 2011 Jul 15;204(2):237-44
pubmed: 21673034
J Virol. 2018 Mar 14;92(7):
pubmed: 29343574
J Med Virol. 1993 Jan;39(1):50-6
pubmed: 7678637
Food Environ Virol. 2015 Mar;7(1):1-6
pubmed: 25326200
Arch Virol. 1997;142(4):637-55
pubmed: 9170494
PLoS Pathog. 2007 May 4;3(5):e64
pubmed: 17480120
J Virol Methods. 2009 Dec;162(1-2):69-74
pubmed: 19646480
J Med Virol. 2009 Nov;81(11):1959-67
pubmed: 19774689
J Clin Virol. 2009 Aug;45(4):292-5
pubmed: 19464943
J Infect Dis. 2005 Aug 15;192(4):658-64
pubmed: 16028135
Ann Neurol. 2006 Aug;60(2):162-73
pubmed: 16862584
Viruses. 2017 Nov 03;9(11):
pubmed: 29099746
J Infect. 2014 Jan;68 Suppl 1:S2-8
pubmed: 24119828
Future Microbiol. 2018 Dec;13:1719-1730
pubmed: 30484707
Brief Bioinform. 2008 Jul;9(4):299-306
pubmed: 18417537
Lancet. 1971 Jun 19;1(7712):1253-7
pubmed: 4104714
Virol J. 2012 Sep 18;9:209
pubmed: 22988938
J Exp Med. 2008 Apr 14;205(4):841-52
pubmed: 18347101
J Clin Virol. 2009 Feb;44(2):115-8
pubmed: 19157970