Mechanisms of enhanced bacterial endospore inactivation during sterilization by ohmic heating.

Bacillus subtilis / genetics Bacterial Proteins / genetics Electricity Heating Hot Temperature Mutation Spores, Bacterial / genetics Sterilization / methods
Bacillus subtilis spores Differentiation of effects Electrotechnologies Inactivation mechanism Ohmic heating Sterilisation

Journal

Bioelectrochemistry (Amsterdam, Netherlands)
ISSN: 1878-562X
Titre abrégé: Bioelectrochemistry
Pays: Netherlands
ID NLM: 100953583

Informations de publication

Date de publication:
Dec 2019
Historique:
received: 01 07 2019
revised: 24 07 2019
accepted: 24 07 2019
pubmed: 5 8 2019
medline: 27 11 2019
entrez: 5 8 2019
Statut: ppublish

Résumé

During ohmic heating, the electric field may additionally inactivate bacterial endospores. However, the exact mechanism of action is unclear. Thus, a mechanistic study was carried out, investigating the possible target of electric fields inside the spore. Bacillus subtilis spores were heated by conventional and ohmic heating in a capillary system under almost identical thermal conditions. Wild-type (PS533) spores were used, as well as isogenic mutants lacking certain components known for their contribution to spores' heat resistance: small-acid soluble proteins (SASP) protecting DNA (PS578); the coat covering the spore (PS3328); and the spore germination enzyme SleB (FB122(+)). Treatment-dependent release of the spore core's depot of dipicolinic acid (DPA) was further evaluated. Up to 2.4 log

Identifiants

DOI: 10.1016/j.bioelechem.2019.107338 PMID: 31377394
pubmed: 31377394
pii: S1567-5394(19)30422-0
doi: 10.1016/j.bioelechem.2019.107338
pii:
doi:

Substances chimiques

Bacterial Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

107338

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Felix Schottroff (F)

Institute of Food Technology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria; Department of Food, Agricultural and Biological Engineering, Ohio State University, Columbus, OH, USA. Electronic address: felix.schottroff@boku.ac.at.

Taras Pyatkovskyy (T)

Department of Food, Agricultural and Biological Engineering, Ohio State University, Columbus, OH, USA.

Kai Reineke (K)

GNT Europa GmbH, Aachen, Germany.

Peter Setlow (P)

Department of Molecular Biology and Biophysics, UCONN Health, Farmington, CT, USA.

Sudhir K Sastry (SK)

Department of Food, Agricultural and Biological Engineering, Ohio State University, Columbus, OH, USA.

Henry Jaeger (H)

Institute of Food Technology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.

Articles similaires

Atomic view of photosynthetic metabolite permeability pathways and confinement in synthetic carboxysome shells.

Daipayan Sarkar, Christopher Maffeo, Markus Sutter et al.
1.00
Photosynthesis Ribulose-Bisphosphate Carboxylase Carbon Dioxide Molecular Dynamics Simulation Cyanobacteria

Spatial multiplex analysis of lung cancer reveals that regulatory T cells attenuate KRAS-G12C inhibitor-induced immune responses.

Megan Cole, Panayiotis Anastasiou, Claudia Lee et al.
1.00
T-Lymphocytes, Regulatory Lung Neoplasms Proto-Oncogene Proteins p21(ras) Animals Humans

Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis.

Spencer D Shelton, Sara House, Luiza Martins Nascentes Melo et al.
1.00
DNA, Mitochondrial Humans Melanoma Mutation Neoplasm Metastasis

Prevalence and implications of fragile X premutation screening in Thailand.

Areerat Hnoonual, Sunita Kaewfai, Chanin Limwongse et al.
1.00
Humans Fragile X Mental Retardation Protein Thailand Male Female

Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus subtilis › Mechanisms of enhanced bacterial endospore...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Mechanisms of enhanced bacterial endospore...
questionsmedicales.fr › Phénomènes physiques › Phénomènes magnétiques › Phénomènes électromagnétiques › Électricité › Mechanisms of enhanced bacterial endospore...
questionsmedicales.fr › Environnement et santé publique › Environnement › Environnement contrôlé › Chauffage › Mechanisms of enhanced bacterial endospore...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Température élevée › Mechanisms of enhanced bacterial endospore...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Mechanisms of enhanced bacterial endospore...
questionsmedicales.fr › Stades d'organismes › Spores › Spores bactériens › Mechanisms of enhanced bacterial endospore...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pratiques en santé publique › Contrôle des maladies transmissibles › Prévention des infections › Stérilisation › Mechanisms of enhanced bacterial endospore...