Separation and characterization of unknown impurities in latamoxef sodium by LC-Q-TOF MS and a summary of their positive-ion fragmentation regularities.
Impurity
LC-Q-TOF MS
Latamoxef sodium for injection
Positive-ion regularities
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
25 Oct 2019
25 Oct 2019
Historique:
received:
08
05
2019
revised:
23
07
2019
accepted:
24
07
2019
pubmed:
5
8
2019
medline:
28
1
2020
entrez:
5
8
2019
Statut:
ppublish
Résumé
A simple and sensitive method was developed for separation and characterization of seventeen impurities from commercial latamoxef sodium for injection by liquid chromatography combined with electrospray ionization and QTOF mass spectrometer (LC-ESI-QTOF MS). The chromatographic separation was performed on a Boston Green ODS-AQ C18 column (250 mm × 4.6 mm, 5 μm) under gradient mode using binary mobile phase: (A) ammonium acetate (10 mM)-methanol (99:1, v/v) and (B) ammonium acetate (10 mM)-methanol (70:30, v/v). Based on tandem multistage MS and high resolution MS data, the molecular formulas and structures of unknown impurities were inferred. A plausible formation mechanism of impurities was also proposed. In addition, the fragmentation regularity of degraded impurities in positive-ion mode was summarized.
Identifiants
pubmed: 31377652
pii: S0731-7085(19)31113-6
doi: 10.1016/j.jpba.2019.112793
pii:
doi:
Substances chimiques
Acetates
0
Ions
0
Sodium
9NEZ333N27
ammonium acetate
RRE756S6Q2
Moxalactam
VUF6C936Z3
Methanol
Y4S76JWI15
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112793Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.