Genetic Depletion of Class I Odorant Receptors Impacts Perception of Carboxylic Acids.
carboxylic acids
enhancer
monoallelic expression
mouse
odor aversion
odorant receptor
olfaction
olfactory bulb
olfactory epithelium
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
19 08 2019
19 08 2019
Historique:
received:
29
03
2019
revised:
28
05
2019
accepted:
28
06
2019
pubmed:
6
8
2019
medline:
4
8
2020
entrez:
6
8
2019
Statut:
ppublish
Résumé
The mammalian main olfactory pathway detects myriad volatile chemicals using >1,000 odorant receptor (OR) genes, which are organized into two phylogenetically distinct classes (class I and class II). An important question is how these evolutionarily conserved classes contribute to odor perception. Here, we report functional inactivation of a large number of class I ORs in mice via identification and deletion of a local cis-acting enhancer in the class I gene cluster. This manipulation reduced expression of half of the 131 intact class I genes. The resulting class I-depleted mice exhibited a significant reduction in the number of glomeruli responding to carboxylic acids-chemicals associated with microbial action and body odors. These mice also exhibit a change in odor perception marked by a selective loss of behavioral aversion to these compounds. Together, our data demonstrate that class I ORs play a critical role in representing a class of biologically relevant chemosignals.
Identifiants
pubmed: 31378611
pii: S0960-9822(19)30848-6
doi: 10.1016/j.cub.2019.06.085
pmc: PMC8049193
mid: NIHMS1534125
pii:
doi:
Substances chimiques
Carboxylic Acids
0
Receptors, Odorant
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2687-2697.e4Subventions
Organisme : NIDCD NIH HHS
ID : F32 DC000357
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC013576
Pays : United States
Organisme : NIDCD NIH HHS
ID : R03 DC014565
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
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