Limonin ameliorates ulcerative colitis by regulating STAT3/miR-214 signaling pathway.
Animals
Anti-Inflammatory Agents
/ pharmacology
Cell Line
Cell Survival
/ drug effects
Colitis, Ulcerative
/ chemically induced
Colon
/ drug effects
Dextran Sulfate
Epithelial Cells
/ drug effects
Interleukin-10
/ immunology
Interleukin-6
/ genetics
Limonins
/ pharmacology
Male
Mice, Inbred C57BL
MicroRNAs
/ genetics
STAT3 Transcription Factor
/ genetics
Signal Transduction
/ drug effects
DSS
Limonin
PTEN
Ulcerative colitis
miR-214
p-STAT3
Journal
International immunopharmacology
ISSN: 1878-1705
Titre abrégé: Int Immunopharmacol
Pays: Netherlands
ID NLM: 100965259
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
17
02
2019
revised:
08
07
2019
accepted:
17
07
2019
pubmed:
6
8
2019
medline:
15
2
2020
entrez:
6
8
2019
Statut:
ppublish
Résumé
Ulcerative colitis (UC) is a major inflammatory bowel disease (IBD) which has become a global public health problem. Limonin is a triterpenoid extracted from citrus which possesses the capacities to against inflammations and cell apoptosis. However, the efficacy and the underlying mechanisms of limonin in the treatment of UC remain unclear. In this study, we first investigated the therapeutic effects of limonin on dextran sodiumsulfate (DSS)-induced UC in vivo by examining the changes of disease activity index (DAI), the colon length, the colon histology, and cyto/chemokine levels. We found that limonin markedly reduced DAI, intestinal damages, and the levels of pro-inflammatory cytokines, such as TNF-α and IL-6. In vitro, limonin significantly repressed the productions of pro-inflammatory cytokines in cultured normal colonic epithelial cells. Mechanistically, we demonstrated that limonin improved the prognosis of UC mainly through downregulating p-STAT3/miR-214 levels. Collectively, our results suggested that limonin was a novel therapeutic agent and it was expected to be translated into the clinic to improve the prognosis of UC.
Identifiants
pubmed: 31382166
pii: S1567-5769(19)30354-6
doi: 10.1016/j.intimp.2019.105768
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents
0
IL10 protein, mouse
0
Interleukin-6
0
Limonins
0
MicroRNAs
0
Mirn214 microRNA, mouse
0
STAT3 Transcription Factor
0
Stat3 protein, mouse
0
interleukin-6, mouse
0
Interleukin-10
130068-27-8
Dextran Sulfate
9042-14-2
limonin
L0F260866S
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105768Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.