Synaptotagmin-1 enables frequency coding by suppressing asynchronous release in a temperature dependent manner.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 08 2019
05 08 2019
Historique:
received:
01
04
2019
accepted:
17
07
2019
entrez:
7
8
2019
pubmed:
7
8
2019
medline:
24
10
2020
Statut:
epublish
Résumé
To support frequency-coded information transfer, mammalian synapses tightly synchronize neurotransmitter release to action potentials (APs). However, release desynchronizes during AP trains, especially at room temperature. Here we show that suppression of asynchronous release by Synaptotagmin-1 (Syt1), but not release triggering, is highly temperature sensitive, and enhances synchronous release during high-frequency stimulation. In Syt1-deficient synapses, asynchronous release increased with temperature, opposite to wildtype synapses. Mutations in Syt1 C2B-domain polybasic stretch (Syt1 K326Q,K327Q,K331Q) did not affect synchronization during sustained activity, while the previously observed reduced synchronous response to a single AP was confirmed. However, an inflexible linker between the C2-domains (Syt1 9Pro) reduced suppression, without affecting synchronous release upon a single AP. Syt1 9Pro expressing synapses showed impaired synchronization during AP trains, which was rescued by buffering global Ca
Identifiants
pubmed: 31383906
doi: 10.1038/s41598-019-47487-9
pii: 10.1038/s41598-019-47487-9
pmc: PMC6683208
doi:
Substances chimiques
Synaptotagmin I
0
Syt1 protein, mouse
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11341Commentaires et corrections
Type : ErratumIn
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