Activity evaluation of pure and doped zinc oxide nanoparticles against bacterial pathogens and Saccharomyces cerevisiae.
Anti-Infective Agents
/ chemistry
Bacillus subtilis
/ drug effects
Escherichia coli
/ drug effects
Listeria monocytogenes
/ drug effects
Microbial Sensitivity Tests
Microbial Viability
/ drug effects
Nanoparticles
/ chemistry
Saccharomyces cerevisiae
/ drug effects
Staphylococcus aureus
/ drug effects
Zinc Oxide
/ chemistry
Saccharomyces cerevisiae
RAPD
SDS-PAGE
antimicrobial activity
bacterial pathogens
zinc oxide nanoparticles
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
12
02
2019
revised:
11
07
2019
accepted:
25
07
2019
pubmed:
7
8
2019
medline:
18
12
2019
entrez:
7
8
2019
Statut:
ppublish
Résumé
This work aimed to evaluate the antimicrobial activity of pure (ZnO) and doped (ZnMgO) zinc oxide (ZnO) nanoparticles on bacterial pathogens and Saccharomyces cerevisiae to confirm their applicability as an alternative to antibiotics and to estimate their biocompatibility. Microbial growth inhibition on agar plates, microbial viability and adaptation tests in broth with ZnO nanoparticles, spore germination, random amplified polymorphic DNA and SDS-PAGE analysis were conducted to evaluate the effects of ZnO nanoparticles on cell morphology, viability, DNA damage and protein production. For this purpose, Escherichia coli, Salmonella, Listeria monocytogenes, Staphylococcus aureus, Bacillus subtilis and S. cerevisiae were studied after the addition of ZnO nanoparticles to the growth media. The contact with ZnO nanoparticles produced changes in morphology, shape, viability, DNA arrangement (DNA fingerprints) and protein content (SDS-PAGE) in treated cells. As reported in this study, ZnO nanoparticles have an antimicrobial effect on both prokaryotic and eukaryotic cells. Before using ZnO nanoparticles as antimicrobial agents, it is important to evaluate the target because their effect depends on their composition, size and dose. We believe that the results obtained can help to optimize manufactured metal oxide nanoparticles in terms of their composition, size and working concentration. The parameters obtained directly define the applicability and biocompatibility of ZnO nanoparticles and thus are essential for any utilization in food, medicine and industry where pathogen control is crucial.
Substances chimiques
Anti-Infective Agents
0
Zinc Oxide
SOI2LOH54Z
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1391-1402Informations de copyright
© 2019 The Society for Applied Microbiology.
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