Two novel lipases purified from rice bran displaying lipolytic and esterification activities.

Chromatography, Gel Enzyme Activation Esterification Hydrogen-Ion Concentration Kinetics Lipase / chemistry Lipolysis Metals Molecular Weight Oryza / enzymology Retinoblastoma-Like Protein p107 / chemistry Retinoblastoma-Like Protein p130 / chemistry Solvents Substrate Specificity Temperature
Esterification Lipase Rice bran

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 Oct 2019
Historique:
received: 05 07 2019
revised: 02 08 2019
accepted: 02 08 2019
pubmed: 7 8 2019
medline: 9 4 2020
entrez: 7 8 2019
Statut: ppublish

Résumé

In this study, two novel lipases, rice bran lipase 1 (RBL1) and rice bran lipase 2 (RBL2), were first identified in rice (Oryza sativa) bran. Through the purification by ammonium sulfate precipitation, ion-exchange chromatography and size-exclusion chromatography, RBL1 and RBL2 were purified to 36- and 339-fold with the final specific activity of 4.73 and 44.06 U/mg, respectively. The purified RBL1 and RBL2 had the molecular weight of 18.8 and 35.5 kDa, respectively. The K

Identifiants

DOI: 10.1016/j.ijbiomac.2019.08.026 PMID: 31386870
pubmed: 31386870
pii: S0141-8130(19)35140-2
doi: 10.1016/j.ijbiomac.2019.08.026
pii:
doi:

Substances chimiques

Metals 0
Retinoblastoma-Like Protein p107 0
Retinoblastoma-Like Protein p130 0
Solvents 0
Lipase EC 3.1.1.3

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

298-306

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Chin-Chung Chen (CC)

Department of Biotechnology, Green Technology Research Institute, CPC Corporation, Taiwan, 521 Sec. 2, Bo-Ai Rd., Chiayi City, Taiwan. Electronic address: 078654@cpc.com.tw.

Guo-Jhan Gao (GJ)

Department of Biotechnology, Green Technology Research Institute, CPC Corporation, Taiwan, 521 Sec. 2, Bo-Ai Rd., Chiayi City, Taiwan. Electronic address: 246905@cpc.com.tw.

Ai-Ling Kao (AL)

Department of Biotechnology, Green Technology Research Institute, CPC Corporation, Taiwan, 521 Sec. 2, Bo-Ai Rd., Chiayi City, Taiwan. Electronic address: 295663@cpc.com.tw.

Chang-Ting Tsai (CT)

Department of Biotechnology, Green Technology Research Institute, CPC Corporation, Taiwan, 521 Sec. 2, Bo-Ai Rd., Chiayi City, Taiwan. Electronic address: 295647@cpc.com.tw.

Zheng-Chia Tsai (ZC)

Department of Biotechnology, Green Technology Research Institute, CPC Corporation, Taiwan, 521 Sec. 2, Bo-Ai Rd., Chiayi City, Taiwan. Electronic address: 078531@cpc.com.tw.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie sur gel › Two novel lipases purified from rice bran...
questionsmedicales.fr › Métabolisme › Activation enzymatique › Two novel lipases purified from rice bran...
questionsmedicales.fr › Métabolisme › Estérification › Two novel lipases purified from rice bran...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Two novel lipases purified from rice bran...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Two novel lipases purified from rice bran...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Carboxylic ester hydrolases › Triacylglycerol lipase › Two novel lipases purified from rice bran...
questionsmedicales.fr › Métabolisme › Métabolisme lipidique › Lipolyse › Two novel lipases purified from rice bran...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Two novel lipases purified from rice bran...
questionsmedicales.fr › Phénomènes chimiques › Masse moléculaire › Two novel lipases purified from rice bran...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Oryza › Two novel lipases purified from rice bran...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Phosphoprotéines › Protéine p107 de type rétinoblastome › Two novel lipases purified from rice bran...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Phosphoprotéines › Protéine p130 de type rétinoblastome › Two novel lipases purified from rice bran...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Solvants › Two novel lipases purified from rice bran...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Two novel lipases purified from rice bran...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Two novel lipases purified from rice bran...