Epithelial alarmin levels in exhaled breath condensate in patients with idiopathic pulmonary fibrosis: A pilot study.
Aged
Alarmins
/ metabolism
Asthma
/ metabolism
Breath Tests
/ methods
Cytokines
/ metabolism
Epithelium
/ metabolism
Exhalation
/ immunology
Female
Humans
Idiopathic Pulmonary Fibrosis
/ diagnosis
Interleukin-17
/ immunology
Interleukin-33
/ immunology
Male
Middle Aged
Pilot Projects
Pulmonary Disease, Chronic Obstructive
/ metabolism
Thymic Stromal Lymphopoietin
IL-25
IL-33
TSLP
epithelial alarmins
exhaled breath condensate
idiopathic pulmonary fibrosis
Journal
The clinical respiratory journal
ISSN: 1752-699X
Titre abrégé: Clin Respir J
Pays: England
ID NLM: 101315570
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
31
05
2019
revised:
30
07
2019
accepted:
01
08
2019
pubmed:
9
8
2019
medline:
12
3
2020
entrez:
9
8
2019
Statut:
ppublish
Résumé
Interleukin (IL)-25, IL-33 and thymic stromal lymphopoietin (TSLP) are epithelial alarmins involved in innate immune responses and have been shown to play an important role in chronic lung diseases. No data are available regarding their levels in exhaled breath condensate (EBC) in idiopathic pulmonary fibrosis (IPF). To examine IL-25, IL-33 and TSLP levels in the EBC obtained from patients with IPF and compare them to those in healthy controls, patients with asthma and chronic obstructive pulmonary disease (COPD). Twenty-three patients with asthma, 25 patients with COPD, 15 patients with IPF and 16 healthy controls were studied. Concentrations of alarmins in the EBC were evaluated by means of ELISA. IL-25 EBC levels were numerically lowest in IPF (25.33 ± 8.84 pg/ml). However, they did not differ significantly from healthy subjects (43.18 ± 5.53 pg/ml), but were significantly lower compared to asthma (72.07 ± 6.03 pg/ml; P < .001). IL-33 EBC levels were significantly increased in IPF (3.41 ± 0.55 pg/ml) compared to healthy controls (1.20 ± 0.60 pg/ml; P < .01) but did not differ from asthma (3.68 pg/ml) and COPD levels (2.47 ± 0.34 pg/ml). There were significant correlations between IL-33 EBC levels and lung diffusion capacity of carbon monoxide (DL IL-25 and IL-33 are detectable in the EBC obtained from IPF subjects. Increased levels of IL-33 compared to healthy controls indicate its possible role in the pathobiology of IPF.
Substances chimiques
Alarmins
0
Cytokines
0
IL25 protein, human
0
IL33 protein, human
0
Interleukin-17
0
Interleukin-33
0
Thymic Stromal Lymphopoietin
GT0IL38SP4
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
652-656Subventions
Organisme : Medical University of Lodz
ID : 503/1-151-03/503-11-002-18
Informations de copyright
© 2019 John Wiley & Sons Ltd.
Références
Saenz SA, Taylor BC, Artis D. Welcome to the neighborhood: epithelial cell-derived cytokines license innate and adaptive immune responses at mucosal sites. Immunol Rev. 2008;226:172-190. https://doi.org/10.1111/j.1600-065X.2008.00713.x.
Xia J, Zhao J, Shang J, et al. Increased IL-33 expression in chronic obstructive pulmonary disease. Am J Physiol Lung Cell Mol Physiol. 2015;308(7):L619-L627. https://doi.org/10.1152/ajplung.00305.2014.
Kim SW, Rhee CK, Kim KU, et al. Factors associated with plasma IL-33 levels in patients with chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis. 2017;12:395-402. https://doi.org/10.2147/COPD.S120445.
Luzina IG, Kopach P, Lockatell V, et al. Interleukin-33 potentiates bleomycin-induced lung injury. Am J Respir Cell Mol Biol. 2013;49(6):999-1008. https://doi.org/10.1165/rcmb.2013-0093OC.
Xu J, Zheng J, Song P, Zhou Y, Guan S. IL-33/ST2 pathway in a bleomycin-induced pulmonary fibrosis model. Mol Med Rep. 2016;14(2):1704-1708. https://doi.org/10.3892/mmr.2016.5446.
Lee J-U, Chang HS, Lee HJ, et al. Upregulation of interleukin-33 and thymic stromal lymphopoietin levels in the lungs of idiopathic pulmonary fibrosis. BMC Pulm Med. 2017;17(1):39. https://doi.org/10.1186/s12890-017-0380-z.
Davis MD, Montpetit A, Hunt J. Exhaled breath condensate: an overview. Immunol Allergy Clin North Am. 2012;32(3):363-375. https://doi.org/10.1016/j.iac.2012.06.014.
Raghu G, Collard HR, Egan JJ, et al. An official ATS/ERS/JRS/ALAT statement: idiopathic pulmonary fibrosis: evidence-based guidelines for diagnosis and management. Am J Respir Crit Care Med. 2011;183(6):788-824. https://doi.org/10.1164/rccm.2009-040GL.
Global Initiative for Asthma (GINA). Global strategy for asthma management and prevention report. Updated 2017. http://www.ginasthma.org.
GOLD 2017 Global Strategy for the Diagnosis, Management and Prevention of COPD. Glob Initiat Chronic Obstr Lung Dis. https://goldcopd.org/gold-2017-global-strategy-diagnosis-management-prevention-copd/. Accessed August 12, 2018.
Horváth I, Hunt J, Barnes PJ, et al. Exhaled breath condensate: methodological recommendations and unresolved questions. Eur Respir J. 2005;26(3):523-548. https://doi.org/10.1183/09031936.05.00029705.
Hallstrand TS, Hackett TL, Altemeier WA, Matute-Bello G, Hansbro PM, Knight DA. Airway epithelial regulation of pulmonary immune homeostasis and inflammation. Clin Immunol Orlando Fla. 2014;151(1):1-15. https://doi.org/10.1016/j.clim.2013.12.003.
Hewlett JC, Kropski JA, Blackwell TS. Idiopathic pulmonary fibrosis: epithelial-mesenchymal interactions and emerging therapeutic targets. Matrix Biol J Int Soc Matrix Biol. 2018;71-72:112-127. https://doi.org/10.1016/j.matbio.2018.03.021.
Li D, Guabiraba R, Besnard A-G, et al. IL-33 promotes ST2-dependent lung fibrosis by the induction of alternatively activated macrophages and innate lymphoid cells in mice. J Allergy Clin Immunol. 2014;134(6):1422-1432.e11. https://doi.org/10.1016/j.jaci.2014.05.011.
Zhao Y, De Los Santos FG, Wu Z, Liu T, Phan SH. An ST2-dependent role of bone marrow-derived group 2 innate lymphoid cells in pulmonary fibrosis. J Pathol. 2018;245(4):399-409. https://doi.org/10.1002/path.5092.
Luzina IG, Pickering EM, Kopach P, et al. Full-length IL-33 promotes inflammation but not Th2 response in vivo in an ST2-independent fashion. J Immunol Baltim Md 1950. 2012;189(1):403-410. https://doi.org/10.4049/jimmunol.1200259.
Datta A, Alexander R, Sulikowski MG, et al. Evidence for a functional thymic stromal lymphopoietin signaling axis in fibrotic lung disease. J Immunol Baltim Md 1950. 2013;191(9):4867-4879. https://doi.org/10.4049/jimmunol.1300588.
Gorska K, Nejman-Gryz P, Paplinska-Goryca M, Korczynski P, Prochorec-Sobieszek M, Krenke R. Comparative study of IL-33 and IL-6 levels in different respiratory samples in mild-to-moderate asthma and COPD. COPD. 2018;15(1):36-45. https://doi.org/10.1080/15412555.2017.1416074.
Hams E, Armstrong ME, Barlow JL, et al. IL-25 and type 2 innate lymphoid cells induce pulmonary fibrosis. Proc Natl Acad Sci U S A. 2014;111(1):367-372. https://doi.org/10.1073/pnas.1315854111.
Majewski S, Tworek D, Szewczyk K, et al. Epithelial alarmin levels in exhaled breath condensate in patients with idiopathic pulmonary fibrosis. Eur Respir J. 2018;52(suppl 62):PA2927. https://doi.org/10.1183/13993003.congress-2018.PA2927.