Telomerase RNAs in land plants.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
10 10 2019
10 10 2019
Historique:
accepted:
30
07
2019
revised:
26
07
2019
received:
11
07
2019
pubmed:
9
8
2019
medline:
4
12
2019
entrez:
9
8
2019
Statut:
ppublish
Résumé
To elucidate the molecular nature of evolutionary changes of telomeres in the plant order Asparagales, we aimed to characterize telomerase RNA subunits (TRs) in these plants. The unusually long telomere repeat unit in Allium plants (12 nt) allowed us to identify TRs in transcriptomic data of representative species of the Allium genus. Orthologous TRs were then identified in Asparagales plants harbouring telomere DNA composed of TTAGGG (human type) or TTTAGGG (Arabidopsis-type) repeats. Further, we identified TRs across the land plant phylogeny, including common model plants, crop plants, and plants with unusual telomeres. Several lines of functional testing demonstrate the templating telomerase function of the identified TRs and disprove a functionality of the only previously reported plant telomerase RNA in Arabidopsis thaliana. Importantly, our results change the existing paradigm in plant telomere biology which has been based on the existence of a relatively conserved telomerase reverse transcriptase subunit (TERT) associating with highly divergent TRs even between closely related plant taxa. The finding of a monophyletic origin of genuine TRs across land plants opens the possibility to identify TRs directly in transcriptomic or genomic data and/or predict telomere sequences synthesized according to the respective TR template region.
Identifiants
pubmed: 31392988
pii: 5545009
doi: 10.1093/nar/gkz695
pmc: PMC6765143
doi:
Substances chimiques
telomerase RNA
0
RNA
63231-63-0
Telomerase
EC 2.7.7.49
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9842-9856Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
Mol Gen Genet. 1998 Dec;260(5):470-4
pubmed: 9894917
Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):73-78
pubmed: 21164032
Proc Biol Sci. 2001 Aug 7;268(1476):1541-6
pubmed: 11487399
Plant J. 2019 Apr;98(2):195-212
pubmed: 30834599
Chromosoma. 2003 Dec;112(4):164-72
pubmed: 14530986
Nucleic Acids Res. 2002 Jul 15;30(14):3059-66
pubmed: 12136088
Science. 2001 Mar 2;291(5509):1797-800
pubmed: 11230697
Curr Genet. 1998 Feb;33(2):83-91
pubmed: 9506895
Plant Cell. 2011 Dec;23(12):4266-79
pubmed: 22202891
Nat Biotechnol. 2011 May 15;29(7):644-52
pubmed: 21572440
Wiley Interdiscip Rev RNA. 2018 Mar;9(2):
pubmed: 29124890
Sci Rep. 2018 Aug 15;8(1):12182
pubmed: 30111865
FEBS Lett. 1999 Sep 3;457(3):465-9
pubmed: 10471830
Nucleic Acids Res. 2016 Nov 16;44(20):9891-9901
pubmed: 27378779
Cell. 1985 Dec;43(2 Pt 1):405-13
pubmed: 3907856
Chromosoma. 2000 Jun;109(3):201-5
pubmed: 10929199
Gigascience. 2014 Oct 27;3:17
pubmed: 25625010
Plant Mol Biol. 2008 Apr;66(6):637-46
pubmed: 18239859
Chromosome Res. 2002;10(2):155-64
pubmed: 11993936
Science. 2003 Aug 1;301(5633):653-7
pubmed: 12893945
Plant Cell Physiol. 2019 Feb 1;60(2):421-435
pubmed: 30462304
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14422-7
pubmed: 8962067
Chromosome Res. 1996 Apr;4(3):207-13
pubmed: 8793205
Am J Bot. 2006 Jun;93(6):814-23
pubmed: 21642143
FEBS Lett. 1996 Aug 12;391(3):307-9
pubmed: 8764995
Plant J. 1999 Dec;20(5):553-61
pubmed: 10652127
Plant J. 2016 Feb;85(3):337-47
pubmed: 26716914
RNA Biol. 2012 Mar;9(3):302-13
pubmed: 22336715
J Mol Biol. 1992 Sep 5;227(1):114-21
pubmed: 1522583
PLoS One. 2012;7(11):e50226
pubmed: 23185583
PLoS Genet. 2015 Jun 15;11(6):e1005281
pubmed: 26075395
Cell. 2018 May 17;173(5):1179-1190.e13
pubmed: 29775593
Nature. 1989 Jan 26;337(6205):331-7
pubmed: 2463488
Genome Biol Evol. 2012;4(3):248-64
pubmed: 22247428
J Exp Bot. 2011 Nov;62(15):5531-45
pubmed: 21865176
Curr Biol. 2014 Feb 17;24(4):465-70
pubmed: 24508168
Plant Sci. 2019 Apr;281:41-51
pubmed: 30824060
Cell. 1988 Apr 8;53(1):127-36
pubmed: 3349525
Proc Biol Sci. 2003 Sep 22;270(1527):1893-904
pubmed: 14561302
Plant J. 2010 Feb 1;61(3):519-28
pubmed: 19891705
Plant J. 2015 May;82(4):644-54
pubmed: 25828846
J Mol Biol. 1994 Jun 3;239(2):163-9
pubmed: 8196051
Genome Biol Evol. 2013;5(3):468-83
pubmed: 23395982
Cells. 2019 Jan 16;8(1):
pubmed: 30654521
RNA. 2007 Nov;13(11):1923-39
pubmed: 17901154
J Exp Bot. 2012 Jun;63(11):4233-41
pubmed: 22511802
Plant J. 2003 May;34(3):283-91
pubmed: 12713535
Front Genet. 2012 Sep 24;3:188
pubmed: 23015808
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
pubmed: 9254694
Chromosome Res. 2005;13(5):469-79
pubmed: 16132812
J Comput Biol. 2000 Feb-Apr;7(1-2):203-14
pubmed: 10890397
Plant Mol Biol. 2006 Mar;60(5):633-46
pubmed: 16649103
Proc Natl Acad Sci U S A. 1989 Sep;86(18):7049-53
pubmed: 2780561
Biol Direct. 2006 Aug 14;1:22
pubmed: 16907971
EMBO Rep. 2002 Dec;3(12):1152-7
pubmed: 12446565
Chromosome Res. 2012 May;20(4):381-94
pubmed: 22543812
RNA Biol. 2016 Aug 2;13(8):720-32
pubmed: 27359343
Proc Natl Acad Sci U S A. 2014 Nov 11;111(45):E4859-68
pubmed: 25355905
Nature. 2018 May;557(7704):190-195
pubmed: 29695869
RNA. 2009 Apr;15(4):546-59
pubmed: 19223441
Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):E693-9
pubmed: 25646484
Annu Rev Biophys. 2017 May 22;46:199-225
pubmed: 28301767
Plant J. 2015 Dec;84(6):1087-99
pubmed: 26485466
Bioinformatics. 2014 Jun 15;30(12):1660-6
pubmed: 24532719
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
RNA. 2012 Oct;18(10):1747-59
pubmed: 22875809