Citrus exocortis viroid causes ribosomal stress in tomato plants.
Citrus
/ virology
Host-Pathogen Interactions
/ genetics
Solanum lycopersicum
/ genetics
Organelle Biogenesis
Plant Diseases
/ genetics
Plant Leaves
/ genetics
Protein Biosynthesis
RNA Interference
RNA, Plant
/ antagonists & inhibitors
RNA, Ribosomal, 18S
/ antagonists & inhibitors
RNA, Viral
/ genetics
Ribosomal Proteins
/ genetics
Ribosomes
/ genetics
Stress, Physiological
/ genetics
Viroids
/ genetics
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
19 09 2019
19 09 2019
Historique:
accepted:
30
07
2019
revised:
23
07
2019
received:
04
06
2019
pubmed:
9
8
2019
medline:
4
12
2019
entrez:
9
8
2019
Statut:
ppublish
Résumé
Viroids are naked RNAs that do not code for any known protein and yet are able to infect plants causing severe diseases. Because of their RNA nature, many studies have focused on the involvement of viroids in RNA-mediated gene silencing as being their pathogenesis mechanism. Here, the alterations caused by the Citrus exocortis viroid (CEVd) on the tomato translation machinery were studied as a new aspect of viroid pathogenesis. The presence of viroids in the ribosomal fractions of infected tomato plants was detected. More precisely, CEVd and its derived viroid small RNAs were found to co-sediment with tomato ribosomes in vivo, and to provoke changes in the global polysome profiles, particularly in the 40S ribosomal subunit accumulation. Additionally, the viroid caused alterations in ribosome biogenesis in the infected tomato plants, affecting the 18S rRNA maturation process. A higher expression level of the ribosomal stress mediator NAC082 was also detected in the CEVd-infected tomato leaves. Both the alterations in the rRNA processing and the induction of NAC082 correlate with the degree of viroid symptomatology. Taken together, these results suggest that CEVd is responsible for defective ribosome biogenesis in tomato, thereby interfering with the translation machinery and, therefore, causing ribosomal stress.
Identifiants
pubmed: 31392997
pii: 5545002
doi: 10.1093/nar/gkz679
pmc: PMC6895259
doi:
Substances chimiques
RNA, Plant
0
RNA, Ribosomal, 18S
0
RNA, Viral
0
Ribosomal Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8649-8661Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
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