Characterization of de novo lytic infection of dermal lymphatic microvascular endothelial cells by Kaposi's sarcoma-associated herpesvirus.
Herpesvirus
KSHV
Latent genes
Lymphatic endothelial cells
Lytic infection
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
12
05
2019
revised:
21
07
2019
accepted:
29
07
2019
pubmed:
9
8
2019
medline:
9
4
2020
entrez:
9
8
2019
Statut:
ppublish
Résumé
The biology of primary lytic Kaposi's sarcoma-associated herpesvirus (KSHV) infection is still not well understood, which is largely attributed to the lack of cell lines permissive to robust lytic KSHV infection in vitro. Our study demonstrates that primary human dermal lymphatic microvascular endothelial cells (HDLMEC) support lytic KSHV replication following de novo infection, resulting in robust KSHV production, indicating that HDLMECs are suitable for studying the regulation of primary lytic KSHV infection. Importantly, by utilizing lytically infected HDLMECs, we show for the first time that the KSHV latent genes LANA and viral cyclin are required for lytic replication during de novo lytic infection, a function of these latent genes that has not yet been recognized. Since Kaposi's sarcoma is considered to be originated from infected lymphatic endothelial cells, HDLMECs represent a valuable in vitro cell culture model for investigating lytic KSHV infection, which has been understudied in KSHV pathogenesis.
Identifiants
pubmed: 31394409
pii: S0042-6822(19)30204-1
doi: 10.1016/j.virol.2019.07.028
pmc: PMC6733618
mid: NIHMS1536568
pii:
doi:
Substances chimiques
Antigens, Viral
0
Cyclins
0
Nuclear Proteins
0
latency-associated nuclear antigen
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
27-31Subventions
Organisme : NIAID NIH HHS
ID : R01 AI132554
Pays : United States
Organisme : NIDCR NIH HHS
ID : T90 DE021990
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
Références
J Virol. 2003 Sep;77(18):9738-49
pubmed: 12941882
J Virol. 2006 Apr;80(7):3445-58
pubmed: 16537612
Cancer Lett. 2011 Jun 28;305(2):163-74
pubmed: 21493001
PLoS Pathog. 2010 Mar 19;6(3):e1000818
pubmed: 20333249
J Virol. 2019 Feb 19;93(5):
pubmed: 30541837
J Virol. 2009 Sep;83(17):8492-501
pubmed: 19553319
J Virol. 2004 Apr;78(8):4074-84
pubmed: 15047824
J Virol. 2004 Apr;78(7):3601-20
pubmed: 15016882
J Virol. 2008 Jan;82(1):278-90
pubmed: 17942552
J Virol. 2003 Apr;77(7):4205-20
pubmed: 12634378
J Virol. 2004 Sep;78(18):10074-85
pubmed: 15331740
N Engl J Med. 1999 Apr 8;340(14):1063-70
pubmed: 10194235
J Virol. 2003 Jun;77(11):6474-81
pubmed: 12743304
Virology. 2008 Sep 30;379(2):234-44
pubmed: 18684478
J Virol. 2007 Nov;81(21):11957-71
pubmed: 17699571
AIDS. 1996 Sep;10(10):1101-5
pubmed: 8874626
PLoS Pathog. 2014 Jul 17;10(7):e1004240
pubmed: 25033463
Adv Virol. 2011;2011:
pubmed: 21625290
Cell Host Microbe. 2013 Apr 17;13(4):429-40
pubmed: 23601105
Virology. 2017 Dec;512:25-33
pubmed: 28898712
J Clin Invest. 2004 Jan;113(1):124-36
pubmed: 14702116
J Biochem. 2012 Sep;152(3):213-20
pubmed: 22825883
J Virol. 2000 Aug;74(16):7451-61
pubmed: 10906198
Adv Virus Res. 2014;88:111-59
pubmed: 24373311
Viruses. 2014 Oct 23;6(10):4005-23
pubmed: 25341664
J Virol. 2008 May;82(9):4235-49
pubmed: 18305042
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10866-71
pubmed: 9724796
PLoS Pathog. 2016 Sep 08;12(9):e1005878
pubmed: 27606464
Viruses. 2015 Jan 14;7(1):116-53
pubmed: 25594835
J Virol. 2012 Sep;86(18):9708-20
pubmed: 22740391
J Virol. 1997 Mar;71(3):1984-91
pubmed: 9032330
Front Microbiol. 2016 Mar 30;7:334
pubmed: 27065950
Nat Rev Cancer. 2010 Oct;10(10):707-19
pubmed: 20865011
PLoS Pathog. 2014 Jul 10;10(7):e1004154
pubmed: 25010730
J Virol. 2001 Jun;75(12):5614-26
pubmed: 11356969