A Heterochromatin-Specific RNA Export Pathway Facilitates piRNA Production.
Active Transport, Cell Nucleus
/ physiology
Animals
Animals, Genetically Modified
Argonaute Proteins
/ metabolism
Cell Line
Cell Nucleus
/ metabolism
Cytoplasm
/ metabolism
DEAD-box RNA Helicases
/ metabolism
DNA Transposable Elements
Drosophila Proteins
/ metabolism
Drosophila melanogaster
/ metabolism
Gene Silencing
Germ Cells
/ metabolism
Heterochromatin
/ metabolism
Intracellular Signaling Peptides and Proteins
/ metabolism
Karyopherins
/ metabolism
Nucleocytoplasmic Transport Proteins
/ metabolism
RNA, Small Interfering
/ metabolism
RNA-Binding Proteins
/ metabolism
Receptors, Cytoplasmic and Nuclear
/ metabolism
Transcription, Genetic
Exportin 1 Protein
Crm1
Drosophila germline
NXF proteins
RNA export
RNA surveillance
UAP56
heterochromatin
piRNA cluster
piRNA pathway
transposon control
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
08 08 2019
08 08 2019
Historique:
received:
11
03
2019
revised:
18
06
2019
accepted:
29
06
2019
entrez:
10
8
2019
pubmed:
10
8
2019
medline:
10
5
2020
Statut:
ppublish
Résumé
PIWI-interacting RNAs (piRNAs) guide transposon silencing in animals. The 22-30 nt piRNAs are processed in the cytoplasm from long non-coding RNAs that often lack RNA processing hallmarks of export-competent transcripts. By studying how these transcripts achieve nuclear export, we uncover an RNA export pathway specific for piRNA precursors in the Drosophila germline. This pathway requires Nxf3-Nxt1, a variant of the hetero-dimeric mRNA export receptor Nxf1-Nxt1. Nxf3 interacts with UAP56, a nuclear RNA helicase essential for mRNA export, and CG13741/Bootlegger, which recruits Nxf3-Nxt1 and UAP56 to heterochromatic piRNA source loci. Upon RNA cargo binding, Nxf3 achieves nuclear export via the exportin Crm1 and accumulates together with Bootlegger in peri-nuclear nuage, suggesting that after export, Nxf3-Bootlegger delivers precursor transcripts to the piRNA processing sites. These findings indicate that the piRNA pathway bypasses nuclear RNA surveillance systems to export unprocessed transcripts to the cytoplasm, a strategy also exploited by retroviruses.
Identifiants
pubmed: 31398345
pii: S0092-8674(19)30772-X
doi: 10.1016/j.cell.2019.07.007
pii:
doi:
Substances chimiques
Argonaute Proteins
0
Boot protein, Drosophila
0
DNA Transposable Elements
0
Drosophila Proteins
0
Heterochromatin
0
Intracellular Signaling Peptides and Proteins
0
Karyopherins
0
Nucleocytoplasmic Transport Proteins
0
Nxf3 protein, Drosophila
0
Nxt1 protein, Drosophila
0
RNA, Small Interfering
0
RNA-Binding Proteins
0
Receptors, Cytoplasmic and Nuclear
0
Hel25E protein, Drosophila
EC 2.7.7.-
DEAD-box RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
964-979.e20Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.