Optimized nested PCR enhances biological diagnosis and phylogenetic analysis of human parvovirus B19 infections.
Journal
Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
21
05
2019
accepted:
11
07
2019
pubmed:
12
8
2019
medline:
11
10
2019
entrez:
12
8
2019
Statut:
ppublish
Résumé
Diagnosis and epidemiological analysis of human parvovirus B19 (hB19V) infections are essential for disease management in severely ill patients. This study aimed to evaluate the performance of an optimized NS1-VP1u nested PCR for detection and sequencing of viruses in clinical samples using 224 clinical and five reference samples. PCR sensitivity, specificity, and positive and negative predictive values were perfect (100%). While phylogenetic analysis of a 615 bp-long fragment demonstrated that the viruses in all of the samples belonged to genotype 1, this study confirmed that this optimized PCR could detect all known hB19V with high performance.
Identifiants
pubmed: 31401693
doi: 10.1007/s00705-019-04368-w
pii: 10.1007/s00705-019-04368-w
doi:
Substances chimiques
Capsid Proteins
0
DNA, Viral
0
Viral Nonstructural Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM