Bicoid-Dependent Activation of the Target Gene hunchback Requires a Two-Motif Sequence Code in a Specific Basal Promoter.
enhancer
gene regulation
promoter
promoter choice
transcription
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
19 09 2019
19 09 2019
Historique:
received:
16
10
2018
revised:
14
05
2019
accepted:
25
06
2019
pubmed:
14
8
2019
medline:
30
1
2020
entrez:
13
8
2019
Statut:
ppublish
Résumé
In complex genetic loci, individual enhancers interact most often with specific basal promoters. Here we investigate the activation of the Bicoid target gene hunchback (hb), which contains two basal promoters (P1 and P2). Early in embryogenesis, P1 is silent, while P2 is strongly activated. In vivo deletion of P2 does not cause activation of P1, suggesting that P2 contains intrinsic sequence motifs required for activation. We show that a two-motif code (a Zelda binding site plus TATA) is required and sufficient for P2 activation. Zelda sites are present in the promoters of many embryonically expressed genes, but the combination of Zelda plus TATA does not seem to be a general code for early activation or Bicoid-specific activation per se. Because Zelda sites are also found in Bicoid-dependent enhancers, we propose that simultaneous binding to both enhancers and promoters independently synchronizes chromatin accessibility and facilitates correct enhancer-promoter interactions.
Identifiants
pubmed: 31402096
pii: S1097-2765(19)30495-2
doi: 10.1016/j.molcel.2019.06.038
pmc: PMC6754290
mid: NIHMS1533825
pii:
doi:
Substances chimiques
DNA-Binding Proteins
0
Drosophila Proteins
0
Homeodomain Proteins
0
Nuclear Proteins
0
Trans-Activators
0
Transcription Factors
0
bcd protein, Drosophila
0
hb protein, Drosophila
0
zld protein, Drosophila
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1178-1187.e4Subventions
Organisme : NCRR NIH HHS
ID : C06 RR015518
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM051946
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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