Proinflammatory Histidyl-Transfer RNA Synthetase-Specific CD4+ T Cells in the Blood and Lungs of Patients With Idiopathic Inflammatory Myopathies.
Adult
Aged
Aged, 80 and over
Antibodies, Antinuclear
/ blood
Bronchoalveolar Lavage Fluid
/ cytology
CD4-Positive T-Lymphocytes
/ immunology
Female
Histidine-tRNA Ligase
/ immunology
Humans
Interferon-gamma
/ immunology
Interleukin-2
/ immunology
Lung
/ cytology
Lung Diseases, Interstitial
/ immunology
Male
Middle Aged
Monocytes
/ immunology
Myositis
/ blood
Th1 Cells
Journal
Arthritis & rheumatology (Hoboken, N.J.)
ISSN: 2326-5205
Titre abrégé: Arthritis Rheumatol
Pays: United States
ID NLM: 101623795
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
23
11
2018
accepted:
06
08
2019
pubmed:
14
8
2019
medline:
18
3
2020
entrez:
13
8
2019
Statut:
ppublish
Résumé
Autoantibodies targeting histidyl-transfer RNA synthetase (HisRS; anti-Jo-1) are common in the idiopathic inflammatory myopathies (IIMs) and antisynthetase syndrome. This study was undertaken to investigate immunity against HisRS in the blood and lungs of patients with IIM/antisynthetase syndrome. Bronchoalveolar lavage (BAL) fluid, BAL fluid cells, and peripheral blood mononuclear cells (PBMCs) from patients with IIM/antisynthetase syndrome (n = 24) were stimulated with full-length HisRS protein or a HisRS-derived peptide (HisRS In BAL fluid, CD4+ T cells from 3 of 4 patients with IIM/antisynthetase syndrome responded to stimulation with HisRS protein, as measured by the median fold change in CD40L expresssion in stimulated cells compared to unstimulated cells (median fold change 3.6, interquartile range [IQR] 2.7-14.7), and 2 of 3 patients with IIM/antisynthetase syndrome had the highest responses to HisRS The results of this study demonstrate a pronounced presence of HisRS-reactive CD4+ T cells in PBMCs and BAL fluid cells from patients with IIM/antisynthetase syndrome as compared to patients with sarcoidosis and healthy controls. These findings, combined with the presence of anti-Jo-1 autoantibodies in BAL fluid and GC-like structures in the lungs, suggest that immune activation against HisRS might take place within the lungs of patients with IIM/antisynthetase syndrome.
Substances chimiques
Antibodies, Antinuclear
0
IL2 protein, human
0
Interleukin-2
0
Jo-1 antibody
0
Interferon-gamma
82115-62-6
Histidine-tRNA Ligase
EC 6.1.1.21
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
179-191Informations de copyright
© 2019, American College of Rheumatology.
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