Capsid Engineering Overcomes Barriers Toward Adeno-Associated Virus Vector-Mediated Transduction of Endothelial Cells.
Amino Acid Sequence
Capsid
/ chemistry
Cell Differentiation
Dependovirus
/ genetics
Genes, Reporter
Genetic Engineering
/ methods
Genetic Therapy
/ methods
Genetic Vectors
/ chemistry
Green Fluorescent Proteins
/ genetics
HEK293 Cells
HeLa Cells
Human Umbilical Vein Endothelial Cells
/ cytology
Humans
Induced Pluripotent Stem Cells
/ cytology
Peptide Library
Transduction, Genetic
/ methods
AAV vectors
endothelial cells
induced pluripotent stem cells
uncoating
uptake
Journal
Human gene therapy
ISSN: 1557-7422
Titre abrégé: Hum Gene Ther
Pays: United States
ID NLM: 9008950
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
pubmed:
14
8
2019
medline:
28
3
2020
entrez:
14
8
2019
Statut:
ppublish
Résumé
Endothelial cells (EC) are targets in gene therapy and regenerative medicine, but they are inefficiently transduced with adeno-associated virus (AAV) vectors of various serotypes. To identify barriers hampering efficient transduction and to develop an optimized AAV variant for EC transduction, we screened an AAV serotype 2-based peptide display library on primary human macrovascular EC. Using a new high-throughput selection and monitoring protocol, we identified a capsid variant, AAV-V
Identifiants
pubmed: 31407607
doi: 10.1089/hum.2019.027
doi:
Substances chimiques
Peptide Library
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM