Characterization and overproduction of cell-associated cholesterol oxidase ChoD from Streptomyces lavendulae YAKB-15.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 08 2019
14 08 2019
Historique:
received:
01
04
2019
accepted:
25
07
2019
entrez:
16
8
2019
pubmed:
16
8
2019
medline:
23
10
2020
Statut:
epublish
Résumé
Cholesterol oxidases are important enzymes with a wide range of applications from basic research to industry. In this study, we have discovered and described the first cell-associated cholesterol oxidase, ChoD, from Streptomyces lavendulae YAKB-15. This strain is a naturally high producer of ChoD, but only produces ChoD in a complex medium containing whole yeast cells. For characterization of ChoD, we acquired a draft genome sequence of S. lavendulae YAKB-15 and identified a gene product containing a flavin adenine dinucleotide binding motif, which could be responsible for the ChoD activity. The enzymatic activity was confirmed in vitro with histidine tagged ChoD produced in Escherichia coli TOP10, which lead to the determination of basic kinetic parameters with K
Identifiants
pubmed: 31413341
doi: 10.1038/s41598-019-48132-1
pii: 10.1038/s41598-019-48132-1
pmc: PMC6694107
doi:
Substances chimiques
Recombinant Proteins
0
Cholesterol Oxidase
EC 1.1.3.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11850Références
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