Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder.

Adult Autism Spectrum Disorder / metabolism Cellular Reprogramming / genetics Female Flow Cytometry Humans Induced Pluripotent Stem Cells / cytology Kruppel-Like Factor 4 Kruppel-Like Transcription Factors / genetics Leukocytes, Mononuclear / cytology Male Octamer Transcription Factor-3 / genetics SOXB1 Transcription Factors / genetics Sendai virus / genetics Young Adult

Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
08 2019
Historique:
received: 01 06 2019
revised: 19 07 2019
accepted: 25 07 2019
pubmed: 16 8 2019
medline: 28 4 2020
entrez: 16 8 2019
Statut: ppublish

Résumé

We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD.

Identifiants

DOI: 10.1016/j.scr.2019.101516 PMID: 31415975
pubmed: 31415975
pii: S1873-5061(19)30146-1
doi: 10.1016/j.scr.2019.101516
pii:
doi:

Substances chimiques

KLF4 protein, human 0
Kruppel-Like Factor 4 0
Kruppel-Like Transcription Factors 0
Octamer Transcription Factor-3 0
POU5F1 protein, human 0
SOX2 protein, human 0
SOXB1 Transcription Factors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101516

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Kiymet Bozaoglu (K)

Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia.

Yujing Gao (Y)

Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia.

Edouard Stanley (E)

Department of Paediatrics, University of Melbourne, Parkville, Australia; Murdoch Children's Research Institute, Parkville, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia.

Miriam Fanjul-Fernández (M)

Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia; Victorian Clinical Genetics Services, Victoria, Australia.

Natasha J Brown (NJ)

Victorian Clinical Genetics Services, Victoria, Australia.

Kate Pope (K)

Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.

Cherie C Green (CC)

Olga Tennison Autism Research Centre, School of Psychology and Public Health, La Trobe University, Bundoora, Victoria, Australia; Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia.

Katerina Vlahos (K)

Murdoch Children's Research Institute, Parkville, Australia.

Koula Sourris (K)

Murdoch Children's Research Institute, Parkville, Australia.

Melanie Bahlo (M)

The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology, University of Melbourne, Melbourne, Australia.

Martin Delatycki (M)

Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia; Victorian Clinical Genetics Services, Victoria, Australia.

Ingrid Scheffer (I)

Department of Paediatrics, University of Melbourne, Parkville, Australia; Department of Medicine, University of Melbourne, Austin Health, Melbourne, VIC, Australia; Florey Institute, Melbourne, VIC, Australia; Department of Neurology, Royal Children's Hospital, Melbourne, Australia.

Paul J Lockhart (PJ)

Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia. Electronic address: paul.lockhart@mcri.edu.au.

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Classifications MeSH

questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Troubles mentaux › Troubles du développement neurologique › Troubles généralisés du développement de l'enfant › Trouble du spectre autistique › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Phénomènes génétiques › Reprogrammation cellulaire › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription Krüppel-like › Facteur-4 de type Kruppel › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription Krüppel-like › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription à domaine POU › Facteurs de transcription Oct › Facteur de transcription Oct-3 › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription SOX › Facteurs de transcription SOX-B1 › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN de polarité négative › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Respirovirus › Virus Sendai › Generation of seven iPSC lines from peripheral...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Jeune adulte › Generation of seven iPSC lines from peripheral...