Comparative Analysis of Microfluidics Thrombus Formation in Multiple Genetically Modified Mice: Link to Thrombosis and Hemostasis.

arterial thrombus formation bleeding collagen glycoprotein VI microfluidics platelets

Journal

Frontiers in cardiovascular medicine
ISSN: 2297-055X
Titre abrégé: Front Cardiovasc Med
Pays: Switzerland
ID NLM: 101653388

Informations de publication

Date de publication:
2019
Historique:
received: 06 05 2019
accepted: 03 07 2019
entrez: 17 8 2019
pubmed: 17 8 2019
medline: 17 8 2019
Statut: epublish

Résumé

Genetically modified mice are indispensable for establishing the roles of platelets in arterial thrombosis and hemostasis. Microfluidics assays using anticoagulated whole blood are commonly used as integrative proxy tests for platelet function in mice. In the present study, we quantified the changes in collagen-dependent thrombus formation for 38 different strains of (genetically) modified mice, all measured with the same microfluidics chamber. The mice included were deficient in platelet receptors, protein kinases or phosphatases, small GTPases or other signaling or scaffold proteins. By standardized re-analysis of high-resolution microscopic images, detailed information was obtained on altered platelet adhesion, aggregation and/or activation. For a subset of 11 mouse strains, these platelet functions were further evaluated in rhodocytin- and laminin-dependent thrombus formation, thus allowing a comparison of glycoprotein VI (GPVI), C-type lectin-like receptor 2 (CLEC2) and integrin α

Identifiants

pubmed: 31417909
doi: 10.3389/fcvm.2019.00099
pmc: PMC6682619
doi:

Types de publication

Journal Article

Langues

eng

Pagination

99

Subventions

Organisme : British Heart Foundation
ID : RG/15/13/31673
Pays : United Kingdom

Références

J Exp Med. 2001 Feb 19;193(4):459-69
pubmed: 11181698
EMBO J. 2001 May 1;20(9):2120-30
pubmed: 11331578
FASEB J. 2001 Sep;15(11):2019-21
pubmed: 11511514
BMC Bioinformatics. 2003 Jan 13;4:2
pubmed: 12525261
FASEB J. 2003 Apr;17(6):685-7
pubmed: 12586747
Blood. 2003 Dec 1;102(12):4021-7
pubmed: 12893753
Blood. 2004 Feb 15;103(4):1333-41
pubmed: 14563646
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
FASEB J. 2005 May;19(7):825-7
pubmed: 15758040
Arterioscler Thromb Vasc Biol. 2005 Dec;25(12):2673-8
pubmed: 16254207
Blood. 2006 Nov 1;108(9):3045-52
pubmed: 16840732
Mol Cell Biol. 2007 Sep;27(17):6038-52
pubmed: 17576811
Circ Res. 2007 Jun 22;100(12):1673-85
pubmed: 17585075
J Biol Chem. 2007 Sep 7;282(36):25993-6001
pubmed: 17616532
Nat Med. 2007 Sep;13(9):1086-95
pubmed: 17721545
Thromb Haemost. 2007 Nov;98(5):1072-80
pubmed: 18000613
Arterioscler Thromb Vasc Biol. 2008 Mar;28(3):403-12
pubmed: 18174460
J Exp Med. 2008 Jul 7;205(7):1583-91
pubmed: 18559454
Biochemistry. 2008 Jul 29;47(30):7831-7
pubmed: 18597489
Pflugers Arch. 2009 Mar;457(5):1173-85
pubmed: 18704487
Blood. 2009 Feb 26;113(9):2056-63
pubmed: 18832659
J Clin Invest. 2009 Feb;119(2):399-407
pubmed: 19147982
Arterioscler Thromb Vasc Biol. 2009 May;29(5):699-705
pubmed: 19213940
Blood. 2009 Aug 27;114(9):1900-3
pubmed: 19549989
Blood. 2009 Oct 15;114(16):3464-72
pubmed: 19641185
J Thromb Haemost. 2010 Mar;8(3):454-62
pubmed: 20002545
Sci Signal. 2010 Jan 05;3(103):ra1
pubmed: 20051593
Blood. 2010 Apr 22;115(16):3364-73
pubmed: 20139097
J Thromb Haemost. 2010 Jun;8(6):1353-63
pubmed: 20230420
J Thromb Haemost. 2010 Jul;8(7):1456-67
pubmed: 20345705
J Biol Chem. 2010 Jul 23;285(30):23410-9
pubmed: 20479008
J Biol Chem. 2010 Jul 30;285(31):23629-38
pubmed: 20519511
J Mol Med (Berl). 2011 Feb;89(2):109-21
pubmed: 21058007
Blood. 2011 Jul 14;118(2):416-24
pubmed: 21527521
J Thromb Haemost. 2011 Jul;9 Suppl 1:44-55
pubmed: 21781241
Blood. 2012 Jan 26;119(4):1054-63
pubmed: 22045984
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Physiol Rev. 2013 Jan;93(1):327-58
pubmed: 23303912
Arterioscler Thromb Vasc Biol. 2013 May;33(5):926-34
pubmed: 23448972
J Biol Chem. 2013 May 10;288(19):13325-36
pubmed: 23519467
Pflugers Arch. 2014 Mar;466(3):407-14
pubmed: 23748496
Circulation. 2013 Jul 30;128(5):541-52
pubmed: 23797810
J Thromb Haemost. 2013 Sep;11(9):1751-9
pubmed: 23815599
Arterioscler Thromb Vasc Biol. 2014 Jun;34(6):1187-92
pubmed: 24675658
Thromb Res. 2014 May;133 Suppl 2:S139-48
pubmed: 24862135
Nat Commun. 2014 Jul 16;5:4257
pubmed: 25027852
Blood. 2014 Sep 25;124(13):2013-24
pubmed: 25115887
Blood. 2015 Jan 1;125(1):144-54
pubmed: 25352128
Nucleic Acids Res. 2015 Jan;43(Database issue):D447-52
pubmed: 25352553
Circulation. 2015 Feb 17;131(7):656-68
pubmed: 25520375
Adv Biol Regul. 2015 Sep;59:36-52
pubmed: 26159296
FASEB J. 2016 Feb;30(2):727-37
pubmed: 26481309
Sci Transl Med. 2017 Apr 5;9(384):
pubmed: 28381538
Blood. 2017 Jun 29;129(26):3407-3418
pubmed: 28465343
Nat Commun. 2017 Jul 13;8:16058
pubmed: 28703137
Blood. 2017 Nov 16;130(20):2171-2179
pubmed: 28972013
Gene. 2018 Feb 5;642:84-94
pubmed: 29129810
Haematologica. 2018 Mar;103(3):540-549
pubmed: 29242293
Blood. 2018 Mar 8;131(10):1122-1144
pubmed: 29301754
Platelets. 2018 Nov;29(7):662-669
pubmed: 29537929
Blood. 2018 Sep 27;132(13):1399-1412
pubmed: 29898956
Blood. 2018 Dec 13;132(24):e35-e46
pubmed: 30275110
Nat Rev Cardiol. 2019 Mar;16(3):166-179
pubmed: 30429532
Cell Calcium. 2019 Jan;77:39-48
pubmed: 30530092
Haematologica. 2019 Jun;104(6):1256-1267
pubmed: 30545925
Nature. 1997 Sep 11;389(6647):183-6
pubmed: 9296496

Auteurs

Magdolna Nagy (M)

Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, Netherlands.

Johanna P van Geffen (JP)

Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, Netherlands.

David Stegner (D)

Rudolf Virchow Center, Institute of Experimental Biomedicine, University Hospital Würzburg, University of Würzburg, Würzburg, Germany.

David J Adams (DJ)

Wellcome Trust Sanger Institute, Cambridge, United Kingdom.

Attila Braun (A)

Rudolf Virchow Center, Institute of Experimental Biomedicine, University Hospital Würzburg, University of Würzburg, Würzburg, Germany.

Susanne M de Witt (SM)

Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, Netherlands.

Margitta Elvers (M)

Department of Vascular Surgery, Experimental Vascular Medicine, Heinrich Heine University, Düsseldorf, Germany.

Mitchell J Geer (MJ)

Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.

Marijke J E Kuijpers (MJE)

Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, Netherlands.

Karl Kunzelmann (K)

Institute of Physiology, University of Regensburg, Regensburg, Germany.

Jun Mori (J)

Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.

Cécile Oury (C)

GIGA-Cardiovascular Sciences, University of Liège, Liège, Belgium.

Joachim Pircher (J)

Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-University, and DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany.

Irina Pleines (I)

Rudolf Virchow Center, Institute of Experimental Biomedicine, University Hospital Würzburg, University of Würzburg, Würzburg, Germany.

Alastair W Poole (AW)

Department of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, Bristol, United Kingdom.

Yotis A Senis (YA)

Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.

Remco Verdoold (R)

Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, Netherlands.

Christian Weber (C)

Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, Netherlands.
Institute for Cardiovascular Prevention (IPEK), Ludwig-Maximilians-Universität München, Munich, Germany.

Bernhard Nieswandt (B)

Rudolf Virchow Center, Institute of Experimental Biomedicine, University Hospital Würzburg, University of Würzburg, Würzburg, Germany.

Johan W M Heemskerk (JWM)

Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, Netherlands.

Constance C F M J Baaten (CCFMJ)

Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, Netherlands.
Institute for Molecular Cardiovascular Research (IMCAR), University Hospital Aachen, Aachen, Germany.

Classifications MeSH