Genomic sequencing of a national emm66 group A streptococci (GAS) outbreak among people who inject drugs and the homeless community in England and Wales, January 2016-May 2017.
Adult
Aged
Aged, 80 and over
Antigens, Bacterial
/ genetics
Bacterial Outer Membrane Proteins
/ genetics
Carrier Proteins
/ genetics
Community-Acquired Infections
/ epidemiology
Disease Outbreaks
Drug Resistance, Bacterial
England
/ epidemiology
Epidemiologic Methods
Female
Ill-Housed Persons
Humans
Male
Middle Aged
Molecular Epidemiology
Streptococcal Infections
/ epidemiology
Streptococcus pyogenes
/ classification
Substance Abuse, Intravenous
/ complications
Wales
/ epidemiology
Whole Genome Sequencing
England
Group A streptococcus
Homeless
Outbreak
People reporting problematic alcohol use
People who inject drugs
Streptococcus pyogenes
Wales
Whole genome sequencing
emm66
Journal
The Journal of infection
ISSN: 1532-2742
Titre abrégé: J Infect
Pays: England
ID NLM: 7908424
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
07
08
2018
revised:
09
08
2019
accepted:
11
08
2019
pubmed:
17
8
2019
medline:
17
7
2020
entrez:
17
8
2019
Statut:
ppublish
Résumé
An outbreak of an uncommon emm type (emm66.0) of group A streptococcus (GAS) occurred in England and Wales between January 2016 and May 2017, involving 52 individuals who were homeless or injecting drugs users. In order to investigate the outbreak, epidemiological and network analysis were performed; moreover 55 isolates (32 outbreak, 5 non-outbreak and 13 historical - 2005-2015) were tested with whole genome sequencing (WGS), antimicrobial resistance determination, Bayesian evolutionary analysis (BEAST). Forty one isolates (including 32 outbreak strains) belonged to a single emm66.0 clade (average SNP difference 6.6; range 0-16 SNPs) separate from the other isolates and two strains previously considered part of the outbreak (SNP average: 5876; range 93-8417 SNPs). Antibiotic resistance was not detected in the outbreak clone. No common source of infection was identified. WGS confirmed expansion of an emm66.0 clone in a hard-to-reach population and enabled refinement of the initial case definition.
Identifiants
pubmed: 31419474
pii: S0163-4453(19)30249-X
doi: 10.1016/j.jinf.2019.08.009
pii:
doi:
Substances chimiques
Antigens, Bacterial
0
Bacterial Outer Membrane Proteins
0
Carrier Proteins
0
streptococcal M protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
435-443Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.