Decreased expression of GPC1 in human skin keratinocytes and epidermis during ageing.
Adult
Aged
Aged, 80 and over
Aging
/ genetics
Cell Proliferation
/ drug effects
Cells, Cultured
Epidermis
/ metabolism
Female
Fibroblast Growth Factor 2
/ pharmacology
Gene Expression Regulation
/ physiology
Glypicans
/ genetics
Humans
Keratinocytes
/ drug effects
Middle Aged
RNA, Messenger
/ genetics
Signal Transduction
/ physiology
Skin
/ metabolism
Young Adult
Ageing
Epidermis
Glypican 1
Human skin
Keratinocytes
Journal
Experimental gerontology
ISSN: 1873-6815
Titre abrégé: Exp Gerontol
Pays: England
ID NLM: 0047061
Informations de publication
Date de publication:
15 10 2019
15 10 2019
Historique:
received:
04
10
2018
revised:
23
07
2019
accepted:
14
08
2019
pubmed:
21
8
2019
medline:
14
7
2020
entrez:
21
8
2019
Statut:
ppublish
Résumé
Glypicans (GPCs) are heparan sulfate cell membrane proteoglycans containing glycosylphosphatidylinositol (GPI) anchor. They play important role in cell behavior by activating/presenting numerous growth factors and cytokines. The expression of GPCs was investigated in primary culture of skin keratinocytes sampled from healthy donors of different age. Primary keratinocytes from healthy female donors aged from 20 to 89 years old (n = 30) were either isolated from breast or abdominal skin samples (n = 27) or purchased (n = 3). GPCs expression was examined by qPCR, immunohistochemistry and western blot. Its role in proliferation induced by fibroblast growth factor 2 (FGF2) was also studied. Glypican 1 (GPC1) was the major expressed GPC in human keratinocytes. Its expression was up to two orders of magnitude higher than other GPCs and was significantly decreased with the age of the donors. It was localized at the cell surface and associated with intracellular granules. In skin sections, GPC1 was mainly localized in basal layer of epidermis. Shedding of GPCs decreased the proliferative effect of FGF2, confirming their role of modulator of growth factor effects on keratinocytes. These results established GPC1 as an important player in epidermis biology and skin ageing.
Sections du résumé
BACKGROUND
Glypicans (GPCs) are heparan sulfate cell membrane proteoglycans containing glycosylphosphatidylinositol (GPI) anchor. They play important role in cell behavior by activating/presenting numerous growth factors and cytokines.
OBJECTIVES
The expression of GPCs was investigated in primary culture of skin keratinocytes sampled from healthy donors of different age.
MATERIALS AND METHODS
Primary keratinocytes from healthy female donors aged from 20 to 89 years old (n = 30) were either isolated from breast or abdominal skin samples (n = 27) or purchased (n = 3). GPCs expression was examined by qPCR, immunohistochemistry and western blot. Its role in proliferation induced by fibroblast growth factor 2 (FGF2) was also studied.
RESULTS
Glypican 1 (GPC1) was the major expressed GPC in human keratinocytes. Its expression was up to two orders of magnitude higher than other GPCs and was significantly decreased with the age of the donors. It was localized at the cell surface and associated with intracellular granules. In skin sections, GPC1 was mainly localized in basal layer of epidermis. Shedding of GPCs decreased the proliferative effect of FGF2, confirming their role of modulator of growth factor effects on keratinocytes. These results established GPC1 as an important player in epidermis biology and skin ageing.
Identifiants
pubmed: 31430521
pii: S0531-5565(18)30634-X
doi: 10.1016/j.exger.2019.110693
pii:
doi:
Substances chimiques
GPC1 protein, human
0
Glypicans
0
RNA, Messenger
0
Fibroblast Growth Factor 2
103107-01-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110693Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.