Compositional and temporal stability of fecal taxon libraries for use with SourceTracker in sub-tropical catchments.

Animals Australia Environmental Monitoring Feces Humans RNA, Ribosomal, 16S Water Microbiology Water Pollution
16S rRNA Amplicon sequencing Microbial source tracking SourceTracker Wastewater

Journal

Water research
ISSN: 1879-2448
Titre abrégé: Water Res
Pays: England
ID NLM: 0105072

Informations de publication

Date de publication:
15 Nov 2019
Historique:
received: 25 03 2019
revised: 06 08 2019
accepted: 08 08 2019
pubmed: 21 8 2019
medline: 26 11 2019
entrez: 21 8 2019
Statut: ppublish

Résumé

Characterization of microbial communities using high-throughput amplicon sequencing is an emerging approach for microbial source tracking of fecal pollution. This study used SourceTracker software to examine temporal and geographical variability of fecal bacterial community profiles to identify pollutant sources in three freshwater catchments in sub-tropical Australia. Fecal bacterial communities from 10 animal species, humans, and composite wastewater samples from six sewage treatment plants were characterized and compared to freshwater samples using Illumina amplicon sequencing of the V5-V6 regions of the 16S rRNA gene. Source contributions were calculated in SourceTracker using new fecal taxon libraries as well as previously generated libraries to determine the effects of geographic and temporal variability on source assignments. SourceTracker determined 16S rRNA bacterial communites within freshwater samples, shared taxonomic similarities to that of wastewater at low levels (typically <3%). SourceTraker also predicted occasional fecal detection of deer and flying fox sources in the water samples. No significant differences in source contributions were observed within sequences from current and previously characterized fecal samples (P ≥ 0.107). However, significant differences were observed between previously characterized and newly characterized source communities (ANOSIM P ≤ 0.001), which shared <15% community composition. Results suggest temporal instability of fecal taxon libraries among tested sources and highlight continual evaluation of community-based MST using confirmatory qPCR analyses of marker genes.

Identifiants

DOI: 10.1016/j.watres.2019.114967 PMID: 31430652
pubmed: 31430652
pii: S0043-1354(19)30741-9
doi: 10.1016/j.watres.2019.114967
pii:
doi:

Substances chimiques

RNA, Ribosomal, 16S 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

114967

Informations de copyright

Crown Copyright © 2019. Published by Elsevier Ltd. All rights reserved.

Auteurs

Christian O'Dea (C)

Genecology Research Centre, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, QLD, 4558, Australia. Electronic address: codea@usc.edu.au.

Qian Zhang (Q)

The Biotechnology Institute, University of Minnesota, MN, 55108, USA. Electronic address: zhangq58@umn.edu.

Christopher Staley (C)

The Biotechnology Institute, University of Minnesota, MN, 55108, USA; Department of Surgery, University of Minnesota, MN, 55455, USA. Electronic address: cmstaley@umn.edu.

Nicole Masters (N)

Genecology Research Centre, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, QLD, 4558, Australia. Electronic address: nmasters@usc.edu.au.

Anna Kuballa (A)

Genecology Research Centre, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, QLD, 4558, Australia. Electronic address: akuballa@usc.edu.au.

Paul Fisher (P)

Seqwater, 117 Brisbane Street, Ipswich, QLD, Australia. Electronic address: paul.fisher@seqwater.com.au.

Cameron Veal (C)

Seqwater, 117 Brisbane Street, Ipswich, QLD, Australia. Electronic address: Cameron.veal@seqwater.com.au.

Helen Stratton (H)

School of Environment and Science, Griffith University, Nathan, QLD, Australia. Electronic address: h.stratton@griffith.edu.au.

Michael J Sadowsky (MJ)

The Biotechnology Institute, University of Minnesota, MN, 55108, USA; Department of Soil, Water, and Climate, University of Minnesota, MN, 55108, USA; Department of Plant and Microbial Biology, University of Minnesota, MN, 55108, USA. Electronic address: sadowsky@umn.edu.

Warish Ahmed (W)

CSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, QLD, Australia. Electronic address: warish.ahmed@csiro.au.

Mohammad Katouli (M)

Genecology Research Centre, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, QLD, 4558, Australia. Electronic address: mkatouli@usc.edu.au.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Compositional and temporal stability of fecal...
questionsmedicales.fr › Régions géographiques › Océanie › Australasie › Australie › Compositional and temporal stability of fecal...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pratiques en santé publique › Surveillance de l'environnement › Compositional and temporal stability of fecal...
questionsmedicales.fr › Liquides et sécrétions biologiques › Fèces › Compositional and temporal stability of fecal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Compositional and temporal stability of fecal...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Compositional and temporal stability of fecal...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Microbiologie de l'environnement › Microbiologie de l'eau › Compositional and temporal stability of fecal...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pollution de l'environnement › Pollution de l'eau › Compositional and temporal stability of fecal...