Novel probes for label-free detection of neurodegenerative GGGGCC repeats associated with amyotrophic lateral sclerosis.
Amyotrophic lateral sclerosis
Electrochemical biosensors
Electrochemical impedance spectroscopy
Nucleic acid biosensing
Repeat expansion disorders
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
30
05
2019
accepted:
06
08
2019
revised:
18
07
2019
pubmed:
23
8
2019
medline:
28
11
2019
entrez:
23
8
2019
Statut:
ppublish
Résumé
DNA repeat expansion sequences cause a myriad of neurological diseases when they expand beyond a critical threshold. Previous electrochemical approaches focused on the detection of trinucleotide repeats (CAG, CGG, and GAA) and relied on labeling of the probe and/or target strands or enzyme-linked assays. However, detection of expanded GC-rich sequences is challenging because they are prone to forming secondary structures such as cruciforms and quadruplexes. Here, we present label-free detection of hexanucleotide GGGGCC repeat sequences, which cause the leading genetic form of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). The approach relies on capturing targets by surface-bound oligonucleotide probes with a different number of complementary repeats, which proportionately translates the length of the target strands into charge transfer resistance (R
Identifiants
pubmed: 31435686
doi: 10.1007/s00216-019-02075-8
pii: 10.1007/s00216-019-02075-8
pmc: PMC7433021
mid: NIHMS1606915
doi:
Substances chimiques
Oligonucleotide Probes
0
RNA
63231-63-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6995-7003Subventions
Organisme : NINDS NIH HHS
ID : R15 NS111374
Pays : United States
Organisme : Amyotrophic Lateral Sclerosis Research Program (ALSRP) grant
ID : Department of Defence
Organisme : NIH HHS
ID : R01 NS100529
Pays : United States
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