The complexity of intercellular localisation of alkaloids revealed by single-cell metabolomics.
Catharanthus roseus
Apocynaceae
Imaging MS
alkaloid
idioblast cell
laticifer cell
secondary metabolism
single-cell MS
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
15
01
2019
accepted:
19
06
2019
pubmed:
23
8
2019
medline:
12
5
2020
entrez:
23
8
2019
Statut:
ppublish
Résumé
Catharanthus roseus is a medicinal plant well known for producing bioactive compounds such as vinblastine and vincristine, which are classified as terpenoid indole alkaloids (TIAs). Although the leaves of this plant are the main source of these antitumour drugs, much remains unknown on how TIAs are biosynthesised from a central precursor, strictosidine, to various TIAs in planta. Here, we have succeeded in showing, for the first time in leaf tissue of C. roseus, cell-specific TIAs localisation and accumulation with 10 μm spatial resolution Imaging mass spectrometry (Imaging MS) and live single-cell mass spectrometry (single-cell MS). These metabolomic studies revealed that most TIA precursors (iridoids) are localised in the epidermal cells, but major TIAs including serpentine and vindoline are localised instead in idioblast cells. Interestingly, the central TIA intermediate strictosidine also accumulates in both epidermal and idioblast cells of C. roseus. Moreover, we also found that vindoline accumulation increases in laticifer cells as the leaf expands. These discoveries highlight the complexity of intercellular localisation in plant specialised metabolism.
Substances chimiques
Secologanin Tryptamine Alkaloids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
848-859Informations de copyright
© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
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