Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius from symptomatic companion animals in Northern Italy: Clonal diversity and novel sequence types.


Journal

Comparative immunology, microbiology and infectious diseases
ISSN: 1878-1667
Titre abrégé: Comp Immunol Microbiol Infect Dis
Pays: England
ID NLM: 7808924

Informations de publication

Date de publication:
Oct 2019
Historique:
received: 14 05 2019
revised: 20 06 2019
accepted: 26 06 2019
pubmed: 23 8 2019
medline: 6 2 2020
entrez: 23 8 2019
Statut: ppublish

Résumé

The aim of this study was to assess the prevalence, the genotypic diversity, the antimicrobial resistance traits of canine and feline clinical methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates in a diagnostic laboratory in Italy during 2015-2016. All isolates were characterized by multilocus sequence typing (MLST), staphylococcal cassette chromosome (SCC)-mec typing and staphylococcal protein A (spa)-typing. The resistance profiles were assessed by antimicrobial susceptibility testing and confirmed genotypically by the detection of mecA gene and by microarray analyses. The prevalence of MRSP isolates was high (31.6%). All the strains were multidrug resistant and the most frequent clone was ST71-SCCmec type II-III. These results confirm a high prevalence of MRSP amongst clinical samples from pets in Italy. These isolates show multidrug resistance features that are of concern both in veterinary and human medicine for clinical and epidemiological reasons.

Identifiants

pubmed: 31437680
pii: S0147-9571(19)30118-3
doi: 10.1016/j.cimid.2019.101331
pii:
doi:

Substances chimiques

Methicillin Q91FH1328A

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

101331

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Maria Luisa Menandro (ML)

Department of Animal Medicine, Productions and Health, University of Padua, Viale dell'Università, 16-35020, Legnaro, PD, Italy. Electronic address: marialuisa.menandro@unipd.it.

Giorgia Dotto (G)

Department of Animal Medicine, Productions and Health, University of Padua, Viale dell'Università, 16-35020, Legnaro, PD, Italy. Electronic address: giorgia.dotto@unipd.it.

Alessandra Mondin (A)

Department of Animal Medicine, Productions and Health, University of Padua, Viale dell'Università, 16-35020, Legnaro, PD, Italy. Electronic address: alessandra.mondin@unipd.it.

Marco Martini (M)

Department of Animal Medicine, Productions and Health, University of Padua, Viale dell'Università, 16-35020, Legnaro, PD, Italy. Electronic address: marco.martini@unipd.it.

Letizia Ceglie (L)

Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università, 10-35020, Legnaro, PD, Italy. Electronic address: lceglie@izsve.it.

Daniela Pasotto (D)

Department of Animal Medicine, Productions and Health, University of Padua, Viale dell'Università, 16-35020, Legnaro, PD, Italy. Electronic address: daniela.pasotto@unipd.it.

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Classifications MeSH