A duplex PCR assay for the simultaneous detection and differentiation of Muscovy duck parvovirus and goose parvovirus.
Detection
Differentiation
Duplex PCR method
Goose parvovirus
Muscovy duck parvovirus
Journal
Molecular and cellular probes
ISSN: 1096-1194
Titre abrégé: Mol Cell Probes
Pays: England
ID NLM: 8709751
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
04
07
2019
revised:
08
08
2019
accepted:
20
08
2019
pubmed:
25
8
2019
medline:
7
5
2020
entrez:
25
8
2019
Statut:
ppublish
Résumé
Both Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) can cause high mortality and morbidity in Muscovy ducklings. MDPVs and GPVs share high nucleotide identity, which can cause errors during differential diagnosis. In this study, the NS genes of both MDPVs and GPVs were chosen for the design of specific primers after genetic comparison. Only three primers (GF1, MF1 and MGR1) were designed for the duplex PCR assay: GF1 is specific for GPV only; MF1 is specific for MDPV only; and MGR1 is highly conserved for both MDPV and GPV. After a series of optimization experiments, the duplex PCR assay amplified a 161-bp fragment specifically for GPV, a 1197-bp fragment specifically for MDPV, and two fragments (161-bp and 1197-bp) for both GPV and MDPV. The lowest detection limit was 10
Identifiants
pubmed: 31445110
pii: S0890-8508(19)30239-7
doi: 10.1016/j.mcp.2019.101439
pii:
doi:
Substances chimiques
Viral Nonstructural Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101439Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.