Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9.
CRISPR
Cas9
Genetics
Transfection
Transgenics
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
28
8
2019
pubmed:
28
8
2019
medline:
24
11
2020
Statut:
ppublish
Résumé
Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.
Identifiants
pubmed: 31452165
doi: 10.1007/978-1-4939-9748-0_13
doi:
Substances chimiques
Luciferases
EC 1.13.12.-
CRISPR-Associated Protein 9
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
219-228Subventions
Organisme : Wellcome Trust
ID : 203134/Z/16/Z
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : K99 AI137442
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI060546
Pays : United States