Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus.
Aquaculture
Biosensors
Food safety
Nuclear magnetic resonance (NMR)
Vibrio parahaemolyticus
Journal
Biomedical journal
ISSN: 2320-2890
Titre abrégé: Biomed J
Pays: United States
ID NLM: 101599820
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
01
12
2017
revised:
30
10
2018
accepted:
22
01
2019
entrez:
31
8
2019
pubmed:
31
8
2019
medline:
19
2
2020
Statut:
ppublish
Résumé
Vibrio parahaemolyticus is a Gram-negative bacterium widely distributed in marine environments and a well-recognized invertebrate pathogen frequently isolated from seafood. V. parahaemolyticus may also spread into humans, via contaminated, raw, or undercooked seafood, causing gastroenteritis and diarrhea. A Nuclear Magnetic Resonance (NMR)-based detection system was used to detect pathogenic levels of this microorganism (10 Compared with conventional microbiology techniques such as real-time PCR (qPCR), the NMR biosensor showed similar limits of detection (LOD) at different concentrations (10 NMR biosensor represents a potential tool for diagnostic and quality control to ensure microbial pathogens such as V. parahaemolyticus are not the cause of infection. The "hybrid" technology (NMR and nanoparticle application) opens a new platform for detecting other microbial pathogens that have impacted human health, animal health and food safety.
Sections du résumé
BACKGROUND
Vibrio parahaemolyticus is a Gram-negative bacterium widely distributed in marine environments and a well-recognized invertebrate pathogen frequently isolated from seafood. V. parahaemolyticus may also spread into humans, via contaminated, raw, or undercooked seafood, causing gastroenteritis and diarrhea.
METHODS
A Nuclear Magnetic Resonance (NMR)-based detection system was used to detect pathogenic levels of this microorganism (10
RESULTS
Compared with conventional microbiology techniques such as real-time PCR (qPCR), the NMR biosensor showed similar limits of detection (LOD) at different concentrations (10
CONCLUSION
NMR biosensor represents a potential tool for diagnostic and quality control to ensure microbial pathogens such as V. parahaemolyticus are not the cause of infection. The "hybrid" technology (NMR and nanoparticle application) opens a new platform for detecting other microbial pathogens that have impacted human health, animal health and food safety.
Identifiants
pubmed: 31466712
pii: S2319-4170(17)30376-1
doi: 10.1016/j.bj.2019.01.009
pmc: PMC6717750
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
187-192Informations de copyright
Copyright © 2019 Chang Gung University. Published by Elsevier B.V. All rights reserved.
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