Identification of a novel antiviral micro-RNA targeting the NS1 protein of the H1N1 pandemic human influenza virus and a corresponding viral escape mutation.
3' Untranslated Regions
Binding Sites
Cell Line, Tumor
Computational Biology
/ methods
Gene Expression Profiling
Genotype
Haplotypes
Host-Pathogen Interactions
/ genetics
Humans
Influenza A Virus, H1N1 Subtype
/ genetics
Influenza, Human
/ epidemiology
MicroRNAs
/ genetics
Mutation
Polymorphism, Genetic
RNA Interference
RNA, Viral
/ genetics
Seasons
Viral Nonstructural Proteins
/ genetics
Antiviral micro-RNA
Escape mutation
Host-pathogen interaction
Human influenza a virus
Influenza pandemics
Journal
Antiviral research
ISSN: 1872-9096
Titre abrégé: Antiviral Res
Pays: Netherlands
ID NLM: 8109699
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
25
03
2019
revised:
22
08
2019
accepted:
25
08
2019
pubmed:
31
8
2019
medline:
9
7
2020
entrez:
31
8
2019
Statut:
ppublish
Résumé
The influenza A virus (IAV) NS1 protein is one of the major regulators of pathogenicity, being able to suppress innate immune response and host protein synthesis. In this study we identified the human micro RNA hsa-miR-1307-3p as a novel potent suppressor of NS1 expression and influenza virus replication. Transcriptomic analysis indicates that hsa-miR-1307-3p also negatively regulates apoptosis and promotes cell proliferation. In addition, we identified a novel mutation in the NS1 gene of A(H1N1)pdm09 strains circulating in Italy in the 2010-11 season, which enabled the virus to escape the hsa-miR-1307-3p inhibition, conferring replicative advantage to the virus in human cells. To the best of our knowledge, this is the first validation of suppression of IAV H1N1 NS1 by a human micro RNA and the first example of an escape mutation from micro RNA-mediated antiviral response for the A(H1N1)pdm09 virus.
Identifiants
pubmed: 31470040
pii: S0166-3542(19)30164-0
doi: 10.1016/j.antiviral.2019.104593
pii:
doi:
Substances chimiques
3' Untranslated Regions
0
INS1 protein, influenza virus
0
MicroRNAs
0
RNA, Viral
0
Viral Nonstructural Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104593Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.