Evaluation of TD test for analysis of persistence or tolerance in clinical isolates of Staphylococcus aureus.
Evaluation
Persistence
Staphylococcus aureus
TD test
Tolerance
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
02
07
2019
revised:
23
08
2019
accepted:
27
08
2019
pubmed:
1
9
2019
medline:
23
7
2020
entrez:
1
9
2019
Statut:
ppublish
Résumé
Besides natural and acquired mechanisms of resistance, bacteria can cope with presence of antibiotics by using complex mechanisms such as persistence or tolerance. The main purpose of this study was to evaluate the suitability of newly developed Tolerance Disk Test (TDtest) (Gefen et al., 2017) to detect persistent or tolerant bacterial cells in clinical isolates of Staphylococcus aureus. The principle of the test is to resuscitate the subpopulation of persistent or tolerant bacterial cells following a disk diffusion test by glucose. Results of the TDtest were evaluated using time killing experiments for three pairs of consecutive S. aureus isolates from lower respiratory airway samples of three cystic fibrosis patients with chronic staphylococcal infections. TDtest enabled semi-quantitative detection of persistent or tolerant bacterial populations in all analyzed isolates for oxacillin, vancomycin, and ciprofloxacin to which isolates studied were susceptible. Therefore, TDtest is a promising method for rapidly determining persistence/tolerance in clinical isolates of S. aureus.
Identifiants
pubmed: 31472196
pii: S0167-7012(19)30561-5
doi: 10.1016/j.mimet.2019.105705
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Glucose
IY9XDZ35W2
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105705Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.