Functional Dissection of a Viral DNA Packaging Machine's Walker B Motif.

AAA Domain / genetics Bacteriophage lambda / enzymology DNA, Viral / chemistry Endodeoxyribonucleases / chemistry Molecular Dynamics Simulation Mutation Nucleoproteins / chemistry Protein Structure, Quaternary Viral Proteins / chemistry Virus Assembly / genetics
molecular motor nucleoprotein packaging complexes terminase virus assembly

Journal

Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R

Informations de publication

Date de publication:
08 11 2019
Historique:
received: 28 06 2019
revised: 09 08 2019
accepted: 19 08 2019
pubmed: 2 9 2019
medline: 17 6 2020
entrez: 2 9 2019
Statut: ppublish

Résumé

Many viruses employ ATP-powered motors for genome packaging. We combined genetic, biochemical, and single-molecule techniques to confirm the predicted Walker-B ATP-binding motif in the phage λ motor and to investigate the roles of the conserved residues. Most changes of the conserved hydrophobic residues resulted in >10

Identifiants

DOI: 10.1016/j.jmb.2019.08.012 PMID: 31473160 PMC: PMC7416571
pubmed: 31473160
pii: S0022-2836(19)30521-2
doi: 10.1016/j.jmb.2019.08.012
pmc: PMC7416571
mid: NIHMS1611860
pii:
doi:

Substances chimiques

DNA, Viral 0
Nucleoproteins 0
Viral Proteins 0
Endodeoxyribonucleases EC 3.1.-
terminase EC 3.1.-

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

4455-4474

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM088186
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM118817
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007343
Pays : United States

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

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Auteurs

Damian delToro (D)

Department of Physics, University of California, San Diego, La Jolla, CA 92093, USA.

David Ortiz (D)

Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA.

Mariam Ordyan (M)

Department of Physics, University of California, San Diego, La Jolla, CA 92093, USA.

Joshua Pajak (J)

Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.

Jean Sippy (J)

Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.

Alexis Catala (A)

Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA.

Choon-Seok Oh (CS)

Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.

Amber Vu (A)

Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.

Gaurav Arya (G)

Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.

Douglas E Smith (DE)

Department of Physics, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: des@ucsd.edu.

Carlos E Catalano (CE)

Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA. Electronic address: carlos.catalano@ucdenver.edu.

Michael Feiss (M)

Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA. Electronic address: michael-feiss@uiowa.edu.

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Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Domaine AAA › Functional Dissection of a Viral DNA Packaging...
questionsmedicales.fr › Virus › Virus à ADN › Caudovirales › Siphoviridae › Bactériophage lambda › Functional Dissection of a Viral DNA Packaging...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN viral › Functional Dissection of a Viral DNA Packaging...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Endonucleases › Endodeoxyribonucleases › Functional Dissection of a Viral DNA Packaging...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de dynamique moléculaire › Functional Dissection of a Viral DNA Packaging...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Functional Dissection of a Viral DNA Packaging...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Functional Dissection of a Viral DNA Packaging...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure quaternaire des protéines › Functional Dissection of a Viral DNA Packaging...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Functional Dissection of a Viral DNA Packaging...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Assemblage viral › Functional Dissection of a Viral DNA Packaging...