A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics.
Anti-Bacterial Agents
/ pharmacology
DNA, Bacterial
/ genetics
Defective Viruses
/ genetics
Diterpenes
/ pharmacology
Drug Resistance, Multiple, Bacterial
/ genetics
Genome, Bacterial
/ genetics
Genomic Islands
/ genetics
Humans
Lincosamides
/ pharmacology
Microbial Sensitivity Tests
Polycyclic Compounds
/ pharmacology
Prophages
/ genetics
Streptococcus pyogenes
/ drug effects
Streptogramin A
/ pharmacology
Whole Genome Sequencing
Pleuromutilins
GAS
L phenotype
LS(A)P phenotype
Streptococcus pyogenes
lnu(B)
lsa(E)
Journal
International journal of antimicrobial agents
ISSN: 1872-7913
Titre abrégé: Int J Antimicrob Agents
Pays: Netherlands
ID NLM: 9111860
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
28
02
2019
revised:
28
06
2019
accepted:
24
08
2019
pubmed:
3
9
2019
medline:
5
3
2020
entrez:
3
9
2019
Statut:
ppublish
Résumé
A lincosamide-resistant and macrolide-susceptible phenotype has not been described to date in Streptococcus pyogenes [group A streptococcus (GAS)]. The aim of this study was to characterize a GAS isolate susceptible to macrolides but resistant to lincosamide, streptogramin A and pleuromutilin antibiotics. Antimicrobial susceptibility was tested using the microdilution broth method and the resistance phenotype was tested by D-test. The GAS2887HUB isolate was subjected to whole-genome sequencing. The isolate showed a positive Gots' test (clindamycin inactivation). Whole-genome sequencing revealed that the strain was ST10 and emm93, and had five resistance genes [lnu(B), ant(6)-Ia, aph(3')-III, tet(M) and dfrG]. The tet(M) gene was located in a Tn916-like transposon. The lsa(E)-lnu(B)-containing sequence (inserted downstream of the rumA gene) was formed by a 39.6-kb prophage, followed by a gene cluster encoding aminoglycoside-streptothricin resistance [ant(6)Ia-sat4-aph(3')III] and lsa(E)-lnu(B) genes. This structure was not transferred by conjugation. This study identified a new genetic element carrying a determinant of lincosamide resistance in a GAS. Further molecular epidemiological surveys are needed to determine the prevalence of this mechanism of resistance in GAS.
Identifiants
pubmed: 31476434
pii: S0924-8579(19)30237-7
doi: 10.1016/j.ijantimicag.2019.08.019
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
DNA, Bacterial
0
Diterpenes
0
Lincosamides
0
Polycyclic Compounds
0
Streptogramin A
8W4UOL59AZ
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
647-651Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.