Evaluation of In-Flow Magnetoresistive Chip Cell-Counter as a Diagnostic Tool.
GMR sensor
diagnostic
whole cell
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
31 Aug 2019
31 Aug 2019
Historique:
received:
07
08
2019
revised:
27
08
2019
accepted:
29
08
2019
entrez:
5
9
2019
pubmed:
5
9
2019
medline:
31
1
2020
Statut:
epublish
Résumé
Inexpensive simple medical devices allowing fast and reliable counting of whole cells are of interest for diagnosis and treatment monitoring. Magnetic-based labs on a chip are one of the possibilities currently studied to address this issue. Giant magnetoresistance (GMR) sensors offer both great sensitivity and device integrability with microfluidics and electronics. When used on a dynamic system, GMR-based biochips are able to detect magnetically labeled individual cells. In this article, a rigorous evaluation of the main characteristics of this magnetic medical device (specificity, sensitivity, time of use and variability) are presented and compared to those of both an ELISA test and a conventional flow cytometer, using an eukaryotic malignant cell line model in physiological conditions (NS1 murine cells in phosphate buffer saline). We describe a proof of specificity of a GMR sensor detection of magnetically labeled cells. The limit of detection of the actual system was shown to be similar to the ELISA one and 10 times higher than the cytometer one.
Identifiants
pubmed: 31480476
pii: bios9030105
doi: 10.3390/bios9030105
pmc: PMC6784370
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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