Fabrication of a high-sensitivity electrochemical immunosensor by the oriented immobilization of engineered nanobody on nanofibrous membrane.
Nanofibers
/ chemistry
Gold
/ chemistry
Biosensing Techniques
/ methods
Electrochemical Techniques
/ methods
Limit of Detection
Single-Domain Antibodies
/ immunology
Metal Nanoparticles
/ chemistry
Immunoassay
/ methods
Antibodies, Immobilized
/ immunology
Electrodes
Polyvinyl Alcohol
/ chemistry
Food Contamination
/ analysis
Membranes, Artificial
Electrochemical impedance spectroscopy
Engineered nanobody
Label-free immunosensor
Modified electrode
Nanofibrous membrane
Oriented immobilization
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
29 10 2024
29 10 2024
Historique:
received:
04
06
2024
accepted:
09
10
2024
medline:
29
10
2024
pubmed:
29
10
2024
entrez:
29
10
2024
Statut:
epublish
Résumé
A new type of label-free electrochemical immunosensor for the high-sensitivity determination of parathion was developed based on the oriented immobilization of nanobody (VHH9) on a gold nanoparticle-loaded polyvinyl alcohol/citric acid nanofiber membrane-modified electrode. The morphology characterization and assembly process of the modified materials were investigated using scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Under the optimum conditions, the label-free electrochemical immunosensor for parathion exhibited a linear range of 0.0015-6400 ng/mL and a low detection limit of 0.48 pg/mL, the signal response of which was 10 times higher than that of the randomly immobilized VHH9. The immunosensor possessed high selectivity, good repeatability and reusability (keeping above 90% of its initial activity after repeating 8 times), and stability (remaining 90% after 9 weeks of storage). Finally, the average recoveries of parathion from food samples were 93.76-105.73% with the coefficient of variation being 2.65-6.85%, showing good correlation with UPLC (R
Identifiants
pubmed: 39470822
doi: 10.1007/s00604-024-06763-w
pii: 10.1007/s00604-024-06763-w
doi:
Substances chimiques
Gold
7440-57-5
Single-Domain Antibodies
0
Antibodies, Immobilized
0
Polyvinyl Alcohol
9002-89-5
Membranes, Artificial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
712Subventions
Organisme : Natural Science Foundation of China
ID : 31972157
Organisme : National Key R&D Program of China
ID : 2019YFE0116600
Organisme : Guangdong Provincial Key Laboratory of Food Quality and Safety
ID : 2020B1212060059
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
Références
Tang Z, Liu X, Su B, et al (2020) Ultrasensitive and rapid detection of ochratoxin A in agro-products by a nanobody-mediated FRET-based immunosensor 387:121678. https://doi.org/10.1016/j.jhazmat.2019.121678
Tang X, Catanante G, Huang X, et al (2022) Screen-printed electrochemical immunosensor based on a novel nanobody for analyzing aflatoxin M1 in milk 383:132598. https://doi.org/10.1016/j.foodchem.2022.132598
Wehmeyer K R, White R J, Kissinger P T, et al (2021) Electrochemical affinity assays/sensors: brief history and current status Vol.14:109. https://doi.org/10.1146/annurev-anchem-061417-125655
Yin W-j, Zhang J-x, Wang H, et al (2023) A highly sensitive electrochemical immunosensor based on electrospun nanocomposite for the detection of parathion 404:134371. https://doi.org/10.1016/j.foodchem.2022.134371
Rahn K L, Peramune U, Zhang T, et al (2023) Label-free electrochemical methods for disease detection 16:49-69. https://doi.org/10.1146/annurev-anchem-091622-085754
Cesewski E, Johnson B N (2020) Electrochemical biosensors for pathogen detection 159:112214. https://doi.org/10.1016/j.bios.2020.112214
Sfragano P S, Moro G, Polo F, et al (2021) The role of peptides in the design of electrochemical biosensors for clinical diagnostics 11. https://doi.org/10.3390/bios11080246
Trilling AK, Hesselink T, Houwelingen AV, et al (2014) Orientation of llama antibodies strongly increases sensitivity of biosensors 60:130-136. https://doi.org/10.1016/j.bios.2014.04.017
Patil A V P V, Chuang Y-S, Li C, et al (2023) Recent advances in electrochemical immunosensors with nanomaterial assistance for signal amplification 13: https://doi.org/10.3390/bios13010125
Biswas S, Lan Q, Xie Y, et al (2021) Label-free electrochemical immunosensor for ultrasensitive detection of carbohydrate antigen 125 based on antibody-immobilized biocompatible MOF-808/CNT 13: 3295–3302. https://doi.org/10.1021/acsami.0c14946
Feng K, Li T, Ye C, et al (2021) A label-free electrochemical immunosensor for rapid detection of salmonella in milk by using CoFe-MOFs-graphene modified electrode 130:108357. https://doi.org/10.1016/j.foodcont.2021.108357
Wu J-H, Hu T-G, Wang H, et al (2022) Electrospinning of PLA nanofibers: recent advances and its potential application for food packaging 70:8207-8221. https://doi.org/10.1021/acs.jafc.2c02611
Kumar V, Vaid K, Bansal S A, et al (2020) Nanomaterial-based immunosensors for ultrasensitive detection of pesticides/herbicides: current status and perspectives 165:112382. https://doi.org/10.1016/j.bios.2020.112382
Sapountzi E, Braiek M, Chateaux J F, et al (2017) Recent advances in electrospun nanofiber interfaces for biosensing devices 17. https://doi.org/10.3390/s17081887
Liu W-X, Song S, Ye M-L, et al (2022) Nanomaterials with excellent adsorption characteristics for sample pretreatment: a review 12. https://doi.org/10.3390/nano12111845
Myndrul V, Coy E, Bechelany M, et al (2021) Photoluminescence label-free immunosensor for the detection of Aflatoxin B1 using polyacrylonitrile/zinc oxide nanofibers 118:111401. https://doi.org/10.1016/j.msec.2020.111401
El-Moghazy A Y, Zhao C, Istamboulie G, et al (2018) Ultrasensitive label-free electrochemical immunosensor based on PVA-co-PE nanofibrous membrane for the detection of chloramphenicol residues in milk 117:838-844. https://doi.org/10.1016/j.bios.2018.07.025
Chauhan D, Solanki P R (2019) Hydrophilic and insoluble electrospun cellulose acetate fiber-based biosensing platform for 25-hydroxy vitamin-D3 detection 1:1613–1623. https://doi.org/10.1021/acsapm.9b00179
Er S, Odaci Demirkol D (2022) Graphene oxide incorporated polystyrene electrospun nanofibers for immunosensing of CD36 as a marker of diabetic plasma 145:108083. https://doi.org/10.1016/j.bioelechem.2022.108083
Yagati AK, Chavan SG, Baek C, et al (2018) Label-free impedance sensing of aflatoxin B1 with polyaniline nanofibers/Au nanoparticle electrode array 18. https://doi.org/10.3390/s18051320
Chauhan D, Gupta PK, Solanki PR (2018) Electrochemical immunosensor based on magnetite nanoparticles incorporated electrospun polyacrylonitrile nanofibers for Vitamin-D3 detection 93:145–156. https://doi.org/10.1016/j.msec.2018.07.036
Huang L, Li Y, Luo C, et al (2022) Novel nanostructure-coupled biosensor platform for one-step high-throughput quantification of serum neutralizing antibody after COVID-19 vaccination 199:113868. https://doi.org/10.1016/j.bios.2021.113868
Simões B, Guedens WJ, Keene C, et al (2021) Direct immobilization of engineered nanobodies on gold sensors 13:17353-17360. https://doi.org/10.1021/acsami.1c02280
Li L, Liu X, He S, et al (2021) Electrochemiluminescence immunosensor based on nanobody and Au/CaCO3 synthesized using waste eggshells for ultrasensitive detection of ochratoxin A 6:30148–30156. https://doi.org/10.1021/acsomega.1c05213
Chen Y, Duan W, Xu L, et al (2022) Nanobody-based label-free photoelectrochemical immunoassay for highly sensitive detection of SARS-CoV-2 spike protein 1211:339904. https://doi.org/10.1016/j.aca.2022.339904
Wen P, Yang Y-Y, Yin W-J, et al (2022) Preparation of an ultrasensitive electrochemical immunosensor for the rapid detection of 19-nortestosterone based on polyvinyl alcohol/polyacrylic acid electrospun nanofiber mat 370:132450. https://doi.org/10.1016/j.snb.2022.132450
Chen D, Yao D, Xie C, et al (2014) Development of an aptasensor for electrochemical detection of tetracycline 42:109-115. https://doi.org/10.1016/j.foodcont.2014.01.018
Guo L, Wang S, He Z, et al (2021) An innovative nanobody-based high-biocompatibility gold interdigitated microelectrode electrochemical bioimpedance sensor for the ultrasensitive detection of difenacoum in human serum 14: https://doi.org/10.3390/ma14143930
Xu L, Xiang H, Chen Z, et al (2019) In situ self-assembly of ultrastable gold nanoparticles on polyvinyl alcohol nanofibrous mats for use as highly reusable catalysts 4:20094-20100. https://doi.org/10.1021/acsomega.9b03436
Lu P, Hsieh Y-L (2010) Multiwalled carbon nanotube (MWCNT) reinforced cellulose fibers by electrospinning 2:2413–2420. https://doi.org/10.1021/am1004128
Mahmoudifard M, Soudi S, Soleimani M, et al (2016) Efficient protein immobilization on polyethersolfone electrospun nanofibrous membrane via covalent binding for biosensing applications 58:586-594. https://doi.org/10.1016/j.msec.2015.09.007
Sonuç Karaboğa MN, Şimşek ÇS, Sezgintürk MK (2016) AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70 84:22-29. https://doi.org/10.1016/j.bios.2015.08.044
Guler Z, Sarac AS (2016) Electrochemical impedance and spectroscopy study of the EDC/NHS activation of the carboxyl groups on poly(ε-caprolactone)/poly(m-anthranilic acid) nanofibers 10:96–110. https://doi.org/10.3144/expresspolymlett.2016.11
Paimard G, Shahlaei M, Moradipour P, et al (2020) An impedimetric immunosensor modified with electrospun core-shell nanofibers for determination of the carcinoma embryonic antigen 311:127928. https://doi.org/10.1016/j.snb.2020.127928
Oloketuyi S, Mazzega E, Zavašnik J, et al (2020) Electrochemical immunosensor functionalized with nanobodies for the detection of the toxic microalgae Alexandrium minutum using glassy carbon electrode modified with gold nanoparticles 154:112052. https://doi.org/10.1016/j.bios.2020.112052
Li H, Yan J, Ou W, et al (2015) Construction of a biotinylated cameloid-like antibody for lable-free detection of apolipoprotein B-100 64:111–118. https://doi.org/10.1016/j.bios.2014.08.060
El-Moghazy AY, Huo J, Amaly N, et al (2020) An innovative nanobody-based electrochemical immunosensor using decorated nylon nanofibers for point-of-care monitoring of human exposure to pyrethroid insecticides 12:6159-6168. https://doi.org/10.1021/acsami.9b16193
Dong X-X, Yang J-Y, Luo L, et al (2017) Portable amperometric immunosensor for histamine detection using Prussian blue-chitosan-gold nanoparticle nanocomposite films 98:305-309. https://doi.org/10.1016/j.bios.2017.07.014
Ou G, Zhao A, Liao H, et al (2023) Au nanopartics decorated urchin-like Bi2S3 on graphene wrapped carbon fiber microelectrode: towards electrochemical immunosensor for sensitive determination of aflatoxin B1 929:117124. https://doi.org/10.1016/j.jelechem.2022.117124