Myosin II isoforms play distinct roles in
adherens junctions
cell biology
cytoskeleton
epithelial cells
mechanobiology
myosin
physics of living systems
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
05 09 2019
05 09 2019
Historique:
received:
06
03
2019
accepted:
05
09
2019
pubmed:
6
9
2019
medline:
12
2
2020
entrez:
6
9
2019
Statut:
epublish
Résumé
Adherens junction (AJ) assembly under force is essential for many biological processes like epithelial monolayer bending, collective cell migration, cell extrusion and wound healing. The acto-myosin cytoskeleton acts as a major force-generator during the de novo formation and remodeling of AJ. Here, we investigated the role of non-muscle myosin II isoforms (NMIIA and NMIIB) in epithelial junction assembly. NMIIA and NMIIB differentially regulate biogenesis of AJ through association with distinct actin networks. Analysis of junction dynamics, actin organization, and mechanical forces of control and knockdown cells for myosins revealed that NMIIA provides the mechanical tugging force necessary for cell-cell junction reinforcement and maintenance. NMIIB is involved in E-cadherin clustering, maintenance of a branched actin layer connecting E-cadherin complexes and perijunctional actin fibres leading to the building-up of anisotropic stress. These data reveal unanticipated complementary functions of NMIIA and NMIIB in the biogenesis and integrity of AJ.
Identifiants
pubmed: 31486768
doi: 10.7554/eLife.46599
pii: 46599
pmc: PMC6756789
doi:
pii:
Substances chimiques
Antigens, CD
0
CDH1 protein, human
0
Cadherins
0
MYH9 protein, human
0
Nonmuscle Myosin Type IIB
EC 3.6.1.-
Myosin Heavy Chains
EC 3.6.4.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Seventh Framework Programme
ID : CoG-617233
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-17-CE13-0013
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-10-INBS-04
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-11-LABX-0071
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-11-LABX-0071
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-17-CE13-0012
Pays : International
Organisme : Ligue Contre le Cancer
ID : Equipe Labellisée
Pays : International
Organisme : Centre National de la Recherche Scientifique
ID : Défi Mechanobiology
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR‐11‐LABX‐0071
Pays : International
Organisme : Ligue Contre le Cancer
ID : Equipe Labellisée
Pays : International
Informations de copyright
© 2019, Heuzé et al.
Déclaration de conflit d'intérêts
MH, GS, JD, VC, TD, DW, JV, PM, RM, BL No competing interests declared
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