AcrAB-TolC efflux pump system plays a role in carbapenem non-susceptibility in Escherichia coli.
Anti-Bacterial Agents
/ pharmacology
Bacterial Proteins
/ metabolism
Carbapenems
/ pharmacology
Carrier Proteins
/ genetics
Drug Resistance, Bacterial
/ drug effects
Escherichia coli
/ drug effects
Escherichia coli Infections
/ microbiology
Escherichia coli Proteins
/ genetics
Humans
India
Microbial Sensitivity Tests
Tertiary Care Centers
Transcription, Genetic
/ drug effects
beta-Lactamases
/ metabolism
AcrAB-TolC
CCCP
Carbapenems
Escherichia coli
Real-time PCR
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
05 09 2019
05 09 2019
Historique:
received:
17
05
2019
accepted:
29
08
2019
entrez:
7
9
2019
pubmed:
7
9
2019
medline:
7
7
2020
Statut:
epublish
Résumé
Efflux pump mediated antibiotic resistance is an unnoticed and undetected mechanism in clinical microbiology laboratory. RND efflux systems are known for aminoglycoside and tetracycline resistance whereas their role in carbapenem non-susceptibility is not established. The study was undertaken to investigate the role of efflux pump in providing resistance against carbapenems and their response against concentration gradient carbapenem stress on the transcriptional level of the AcrAB gene in the clinical isolates of Escherichia coli from a tertiary referral hospital of Northeast India. Out of 298 non-susceptible Escherichia coli isolates 98 isolates were found to have efflux pump mediated carbapenem non-susceptibility. Among them thirty-five were non carbapenemase producers and their expressional levels were verified using qRT-PCR under concentration gradient carbapenem stress. In this study, a strong correlation between ertapenem resistance and AcrA overexpression was observed which has not been reported previously. Further, it was observed that imipenem stress increased AcrB expression in Escherichia coli which holds the novelty of this study. Additionally, the transcription of AcrR was insistently increased which is much higher than the transcriptional level of AcrA under concentration gradient carbapenem stress condition. The study established that AcrAB pump is a relevant antibiotic resistance determinant in bacterial pathogen, has an important role in developing resistance against carbapenem group of antibiotics.
Sections du résumé
BACKGROUND
Efflux pump mediated antibiotic resistance is an unnoticed and undetected mechanism in clinical microbiology laboratory. RND efflux systems are known for aminoglycoside and tetracycline resistance whereas their role in carbapenem non-susceptibility is not established. The study was undertaken to investigate the role of efflux pump in providing resistance against carbapenems and their response against concentration gradient carbapenem stress on the transcriptional level of the AcrAB gene in the clinical isolates of Escherichia coli from a tertiary referral hospital of Northeast India.
RESULTS
Out of 298 non-susceptible Escherichia coli isolates 98 isolates were found to have efflux pump mediated carbapenem non-susceptibility. Among them thirty-five were non carbapenemase producers and their expressional levels were verified using qRT-PCR under concentration gradient carbapenem stress. In this study, a strong correlation between ertapenem resistance and AcrA overexpression was observed which has not been reported previously. Further, it was observed that imipenem stress increased AcrB expression in Escherichia coli which holds the novelty of this study. Additionally, the transcription of AcrR was insistently increased which is much higher than the transcriptional level of AcrA under concentration gradient carbapenem stress condition.
CONCLUSION
The study established that AcrAB pump is a relevant antibiotic resistance determinant in bacterial pathogen, has an important role in developing resistance against carbapenem group of antibiotics.
Identifiants
pubmed: 31488061
doi: 10.1186/s12866-019-1589-1
pii: 10.1186/s12866-019-1589-1
pmc: PMC6727511
doi:
Substances chimiques
AcrAB-TolC protein, E coli
0
Anti-Bacterial Agents
0
Bacterial Proteins
0
Carbapenems
0
Carrier Proteins
0
Escherichia coli Proteins
0
beta-Lactamases
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
210Références
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